Sequence characterization and comparative analysis of three plasmids isolated from environmental Vibrio spp.

Tracy H. Hazen, Dongying Wu, Jonathan A Eisen, Patricia A. Sobecky

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The horizontal transfer of genes by mobile genetic elements such as plasmids and phages can accelerate genome diversification of Vibrio spp., affecting their physiology, pathogenicity, and ecological character. In this study, sequence analysis of three plasmids from Vibrio spp. previously isolated from salt marsh sediment revealed the remarkable diversity of these elements. Plasmids p0908 (81.4 kb), p23023 (52.5 kb), and p09022 (31.0 kb) had a predicted 99, 64, and 32 protein-coding sequences and G+C contents of 49.2%, 44.7%, and 42.4%, respectively. A phylogenetic tree based on concatenation of the host 16S rRNA and rpoA nucleotide sequences indicated p23023 and p09022 were isolated from strains most closely related to V. mediterranei and V. campbellii, respectively, while the host of p0908 forms a clade with V. fluvialis and V. furnissii. Many predicted proteins had amino acid identities to proteins of previously characterized phages and plasmids (24 to 94%). Predicted proteins with similarity to chromosomally encoded proteins included RecA, a nucleoid-associated protein (NdpA), a type IV helicase (UvrD), and multiple hypothetical proteins. Plasmid p0908 had striking similarity to enterobacteria phage P1, sharing genetic organization and amino acid identity for 23 predicted proteins. This study provides evidence of genetic exchange between Vibrio plasmids, phages, and chromosomes among diverse Vibrio spp.

Original languageEnglish (US)
Pages (from-to)7703-7710
Number of pages8
JournalApplied and Environmental Microbiology
Volume73
Issue number23
DOIs
StatePublished - Dec 2007

Fingerprint

Vibrio
plasmid
plasmids
Plasmids
protein
Proteins
Bacteriophages
proteins
bacteriophages
Enterobacteria phages
Bacteriophage P1
Rec A Recombinases
Interspersed Repetitive Sequences
amino acid
Amino Acids
Horizontal Gene Transfer
Wetlands
Base Composition
amino acids
analysis

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology
  • Environmental Science(all)
  • Microbiology

Cite this

Sequence characterization and comparative analysis of three plasmids isolated from environmental Vibrio spp. / Hazen, Tracy H.; Wu, Dongying; Eisen, Jonathan A; Sobecky, Patricia A.

In: Applied and Environmental Microbiology, Vol. 73, No. 23, 12.2007, p. 7703-7710.

Research output: Contribution to journalArticle

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