Sequence analysis and editing of the phosphoprotein (P) gene of rinderpest virus

Miles Yamanaka, Beverly Dale, Tracey Crisp, Barbara Cordell, Marvin Grubman, Tilahun Yilma

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

We have cloned the cDNA of the phosphoprotein (P) gene of the virulent (Kabete "0") strain of rinderpest virus and provided a comparative analysis of its sequence with that of the P genes of measles, canine distemper, and phocid distemper viruses. The gene encodes two overlapping open reading frames of 1521 and 531 nucleotides. Use of the first ATG would produce a P polypeptide of 507 amino acids with a calculated molecular weight of 54,344. The second ATG would produce a C polypeptide of 177 residues with a predicted molecular weight of 19,927. In addition, the insertion of a G residue at position 740 generates an alternative mRNA potentially encoding the V pollypeptide of rinderpest virus. The homology comparisons in P amino acid sequences between rinderpest and measles, between rinderpest and canine distemper, and between rinderpest and phocid distemper viruses are 60, 44, and 46%, respectively. A four-way comparison shows an identity of 34%. Similar homology comparisons with the C amino acid sequence between rinderpest and measles, rinderpest and canine distemper, and rinderpest and phocid distemper viruses are 56, 42, and 40%, respectively. A homology of 31 % is found in a four-way comparison for the C polypeptide. From the point of the insertion of the G residue, there is a homology of 78% between the V polypeptides of rinderpest and measle viruses.

Original languageEnglish (US)
Pages (from-to)553-556
Number of pages4
JournalVirology
Volume190
Issue number1
DOIs
StatePublished - 1992

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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    Yamanaka, M., Dale, B., Crisp, T., Cordell, B., Grubman, M., & Yilma, T. (1992). Sequence analysis and editing of the phosphoprotein (P) gene of rinderpest virus. Virology, 190(1), 553-556. https://doi.org/10.1016/0042-6822(92)91252-P