TY - JOUR
T1 - Separation of three biologically distinct activities from the parasporal crystal of Bacillus thuringiensis var. israelensis
AU - Cheung, Peter Y K
AU - Hammock, Bruce D.
PY - 1985/5
Y1 - 1985/5
N2 - Three fractions showing biologically distinct activities were isolated from the Bacillus thruingiensis var. israelensis crystal δ-endotoxin. Using a shallow sodium chloride gradient on a DEAE anion-exchange column, three pooled fractions were obtained consisting of the 25Kd peptide; 25,26Kd peptides; and 31,34,35Kd peptides, respectively. The 25Kd peptide was hemolytic against human erythrocytes but not mosquitocidal. The 25,26Kd peptide mixture was hemolytic, not mosquitocidal, but toxic to larvae of Trichoplusia ni when injected into the hemocoel of these animals. The 31,34,35Kd peptide mixture was toxic to mosquito larvae, but neither hemolytic nor toxic to larvae of T. ni upon injection. In addition, teratogenic effects were observed when wandering larvae of T. ni were injected with each of these three fractions. All of the biological activities described were destroyed when peptides were treated for 15 s at 95°C or greater. The hemolytic activity was elevated by heating the peptide(s) at 70°C for 15 s. On the other hand, this elevation was not observed with the two insecticidal activities.
AB - Three fractions showing biologically distinct activities were isolated from the Bacillus thruingiensis var. israelensis crystal δ-endotoxin. Using a shallow sodium chloride gradient on a DEAE anion-exchange column, three pooled fractions were obtained consisting of the 25Kd peptide; 25,26Kd peptides; and 31,34,35Kd peptides, respectively. The 25Kd peptide was hemolytic against human erythrocytes but not mosquitocidal. The 25,26Kd peptide mixture was hemolytic, not mosquitocidal, but toxic to larvae of Trichoplusia ni when injected into the hemocoel of these animals. The 31,34,35Kd peptide mixture was toxic to mosquito larvae, but neither hemolytic nor toxic to larvae of T. ni upon injection. In addition, teratogenic effects were observed when wandering larvae of T. ni were injected with each of these three fractions. All of the biological activities described were destroyed when peptides were treated for 15 s at 95°C or greater. The hemolytic activity was elevated by heating the peptide(s) at 70°C for 15 s. On the other hand, this elevation was not observed with the two insecticidal activities.
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U2 - 10.1007/BF01567663
DO - 10.1007/BF01567663
M3 - Article
AN - SCOPUS:0022006953
VL - 12
SP - 121
EP - 126
JO - Current Microbiology
JF - Current Microbiology
SN - 0343-8651
IS - 3
ER -