Sensitivity to the two peptide bacteriocin plantaricin EF is dependent on CorC, a membrane-bound, magnesium/cobalt efflux protein

Dustin D. Heeney, Vladimir Yarov-Yarovoy, Maria L. Marco

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Lactic acid bacteria produce a variety of antimicrobial peptides known as bacteriocins. Most bacteriocins are understood to kill sensitive bacteria through receptor-mediated disruptions. Here, we report on the identification of the Lactobacillus plantarum plantaricin EF (PlnEF) receptor. Spontaneous PlnEF-resistant mutants of the PlnEF-indicator strain L. plantarum NCIMB 700965 (LP965) were isolated and confirmed to maintain cellular ATP levels in the presence of PlnEF. Genome comparisons resulted in the identification of a single mutated gene annotated as the membrane-bound, magnesium/cobalt efflux protein CorC. All isolates contained a valine (V) at position 334 instead of a glycine (G) in a cysteine-β-synthase domain at the C-terminal region of CorC. In silico template-based modeling of this domain indicated that the mutation resides in a loop between two β-strands. The relationship between PlnEF, CorC, and metal homeostasis was supported by the finding that PlnEF-resistance was lost when PlnEF was applied together with high concentrations of Mg2+, Co2+, Zn2+, or Cu2+. Lastly, PlnEF sensitivity was increased upon heterologous expression of LP965 corC but not the G334V CorC mutant in the PlnEF-resistant strain Lactobacillus casei BL23. These results show that PlnEF kills sensitive bacteria by targeting CorC.

Original languageEnglish (US)
Article numbere827
JournalMicrobiologyOpen
Volume8
Issue number11
DOIs
StatePublished - Nov 1 2019

Fingerprint

Bacteriocins
Cobalt
Magnesium
Peptides
Membranes
Proteins
Lactobacillus plantarum
Bacteria
Cysteine Synthase
plantaricin EF
Lactobacillus casei
Valine
Computer Simulation
Glycine
Lactic Acid
Homeostasis
Adenosine Triphosphate
Metals
Genome

Keywords

  • antimicrobial resistance
  • bacteriocins
  • lactic acid bacteria
  • Lactobacillus
  • probiotics

ASJC Scopus subject areas

  • Microbiology

Cite this

Sensitivity to the two peptide bacteriocin plantaricin EF is dependent on CorC, a membrane-bound, magnesium/cobalt efflux protein. / Heeney, Dustin D.; Yarov-Yarovoy, Vladimir; Marco, Maria L.

In: MicrobiologyOpen, Vol. 8, No. 11, e827, 01.11.2019.

Research output: Contribution to journalArticle

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