Sensitivity of fecal occult blood testing in the cat

Adam J. Rudinsky, Julien Guillaumin, Chen Gilor

Research output: Contribution to journalArticle

Abstract

Objectives: The impact of dietary factors on fecal occult blood (FOB) testing has been previously evaluated in cats, but the analytical sensitivity of this point-of-care test remains unexamined. The primary goal of this study was to assess the analytical sensitivity of the FOB test in cats. Methods: Five cats were used in a repeated measures study. Following oral administration of blood, feces were collected and tested every 12 h for FOB and melena. All cats were fed an animal protein-free diet starting the week before entry into the study. Blood was administered on a milligram of hemoglobin per kilogram of body weight basis, and dosed at 1.5, 3, 15, 30 and 45 mg/kg hemoglobin in series with a wash-out period between each trial. Results: FOB was detected in one cat at 1.5 mg/kg hemoglobin, three cats at 3 mg/kg hemoglobin and in all five cats at 15, 30 and 45 mg/kg hemoglobin. Melena was noted in one cat at 30 mg/kg and four cats at 45 mg/kg, but not at lower doses. Conclusions and relevance: Administration of 15 mg/kg hemoglobin (equivalent to about 1.5 ml blood) was sufficient for positive results in all cats. However, detection occurred with as little as 1.5 mg/kg hemoglobin. Thus, FOB has good analytical sensitivity in cats under appropriate clinical situations.

Original languageEnglish (US)
Pages (from-to)603-608
Number of pages6
JournalJournal of Feline Medicine and Surgery
Volume19
Issue number6
DOIs
StatePublished - Jun 1 2017

Fingerprint

Occult Blood
Cats
cats
blood
hemoglobin
Hemoglobins
testing
Melena
detection limit
Point-of-Care Systems
Protein-Restricted Diet
hematologic tests
animal proteins
Hematologic Tests
Feces
diet
oral administration
Oral Administration
feces
Body Weight

ASJC Scopus subject areas

  • Small Animals

Cite this

Sensitivity of fecal occult blood testing in the cat. / Rudinsky, Adam J.; Guillaumin, Julien; Gilor, Chen.

In: Journal of Feline Medicine and Surgery, Vol. 19, No. 6, 01.06.2017, p. 603-608.

Research output: Contribution to journalArticle

Rudinsky, Adam J. ; Guillaumin, Julien ; Gilor, Chen. / Sensitivity of fecal occult blood testing in the cat. In: Journal of Feline Medicine and Surgery. 2017 ; Vol. 19, No. 6. pp. 603-608.
@article{1b9857a359a442af9dc0f0480bbd6280,
title = "Sensitivity of fecal occult blood testing in the cat",
abstract = "Objectives: The impact of dietary factors on fecal occult blood (FOB) testing has been previously evaluated in cats, but the analytical sensitivity of this point-of-care test remains unexamined. The primary goal of this study was to assess the analytical sensitivity of the FOB test in cats. Methods: Five cats were used in a repeated measures study. Following oral administration of blood, feces were collected and tested every 12 h for FOB and melena. All cats were fed an animal protein-free diet starting the week before entry into the study. Blood was administered on a milligram of hemoglobin per kilogram of body weight basis, and dosed at 1.5, 3, 15, 30 and 45 mg/kg hemoglobin in series with a wash-out period between each trial. Results: FOB was detected in one cat at 1.5 mg/kg hemoglobin, three cats at 3 mg/kg hemoglobin and in all five cats at 15, 30 and 45 mg/kg hemoglobin. Melena was noted in one cat at 30 mg/kg and four cats at 45 mg/kg, but not at lower doses. Conclusions and relevance: Administration of 15 mg/kg hemoglobin (equivalent to about 1.5 ml blood) was sufficient for positive results in all cats. However, detection occurred with as little as 1.5 mg/kg hemoglobin. Thus, FOB has good analytical sensitivity in cats under appropriate clinical situations.",
author = "Rudinsky, {Adam J.} and Julien Guillaumin and Chen Gilor",
year = "2017",
month = "6",
day = "1",
doi = "10.1177/1098612X16643752",
language = "English (US)",
volume = "19",
pages = "603--608",
journal = "Journal of Feline Medicine and Surgery",
issn = "1098-612X",
publisher = "W.B. Saunders Ltd",
number = "6",

}

TY - JOUR

T1 - Sensitivity of fecal occult blood testing in the cat

AU - Rudinsky, Adam J.

AU - Guillaumin, Julien

AU - Gilor, Chen

PY - 2017/6/1

Y1 - 2017/6/1

N2 - Objectives: The impact of dietary factors on fecal occult blood (FOB) testing has been previously evaluated in cats, but the analytical sensitivity of this point-of-care test remains unexamined. The primary goal of this study was to assess the analytical sensitivity of the FOB test in cats. Methods: Five cats were used in a repeated measures study. Following oral administration of blood, feces were collected and tested every 12 h for FOB and melena. All cats were fed an animal protein-free diet starting the week before entry into the study. Blood was administered on a milligram of hemoglobin per kilogram of body weight basis, and dosed at 1.5, 3, 15, 30 and 45 mg/kg hemoglobin in series with a wash-out period between each trial. Results: FOB was detected in one cat at 1.5 mg/kg hemoglobin, three cats at 3 mg/kg hemoglobin and in all five cats at 15, 30 and 45 mg/kg hemoglobin. Melena was noted in one cat at 30 mg/kg and four cats at 45 mg/kg, but not at lower doses. Conclusions and relevance: Administration of 15 mg/kg hemoglobin (equivalent to about 1.5 ml blood) was sufficient for positive results in all cats. However, detection occurred with as little as 1.5 mg/kg hemoglobin. Thus, FOB has good analytical sensitivity in cats under appropriate clinical situations.

AB - Objectives: The impact of dietary factors on fecal occult blood (FOB) testing has been previously evaluated in cats, but the analytical sensitivity of this point-of-care test remains unexamined. The primary goal of this study was to assess the analytical sensitivity of the FOB test in cats. Methods: Five cats were used in a repeated measures study. Following oral administration of blood, feces were collected and tested every 12 h for FOB and melena. All cats were fed an animal protein-free diet starting the week before entry into the study. Blood was administered on a milligram of hemoglobin per kilogram of body weight basis, and dosed at 1.5, 3, 15, 30 and 45 mg/kg hemoglobin in series with a wash-out period between each trial. Results: FOB was detected in one cat at 1.5 mg/kg hemoglobin, three cats at 3 mg/kg hemoglobin and in all five cats at 15, 30 and 45 mg/kg hemoglobin. Melena was noted in one cat at 30 mg/kg and four cats at 45 mg/kg, but not at lower doses. Conclusions and relevance: Administration of 15 mg/kg hemoglobin (equivalent to about 1.5 ml blood) was sufficient for positive results in all cats. However, detection occurred with as little as 1.5 mg/kg hemoglobin. Thus, FOB has good analytical sensitivity in cats under appropriate clinical situations.

UR - http://www.scopus.com/inward/record.url?scp=85020109223&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85020109223&partnerID=8YFLogxK

U2 - 10.1177/1098612X16643752

DO - 10.1177/1098612X16643752

M3 - Article

VL - 19

SP - 603

EP - 608

JO - Journal of Feline Medicine and Surgery

JF - Journal of Feline Medicine and Surgery

SN - 1098-612X

IS - 6

ER -