Abstract
Although no animal is a perfect skin model for the study of toxicological and therapeutic agents, structurally the pig may be superior to even non-human primates. Because our work involves effects of toxicological and therapeutic agents on the skin, we wanted to identify stains which may prove useful as well as determine cross-reactivity of some newer antihuman antibodies. We performed a battery of formalin-fixed skin from weanling pigs and minipigs. The battery of antibodies included LCA, CD3, OPD-4, CD34, UCHL- 1, L-26, KP-1, MAC-387, Factor XIIIa, Leu-7, S-100 protein, HMB-45, GFAP, synaptophysin, neurofilament protein, ubiquitin, vimentin, type IV collagen, laminin, fibronectin, Factor VIII related antigen, Desmin-M, smooth muscle actin, cytokeratin 7, cytokeratin 20, AE1/AE3, CAM 5.2, EMA, GCDFP, Ki-67, and PCNA. Immunohistochemical stains for CD3, Leu-7, S-100 protein, type IV collagen, laminin, Factor VIII related antigen, GFAP, synaptophysin, neurofilament protein, ubiquitin, smooth muscle actin, vimentin, Desmin-M, cytokeratin 7, cytokeratin 20, AE1/AE3, CAM 5.2, Ki-67 and PCNA showed consistent cross-reactivity. In formalin-fixed tissue, only antibodies to lymphoreticular cells showed poor cross-reactivity. A high percentage of the remaining antibodies did show good cross-reactivity but with some interesting similarities and differences in specificity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 19-29 |
| Number of pages | 11 |
| Journal | Journal of Dermatological Science |
| Volume | 18 |
| Issue number | 1 |
| DOIs | |
| State | Published - Sep 1998 |
| Externally published | Yes |
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Keywords
- Animal model
- Immunohistochemical stains
- Pig
ASJC Scopus subject areas
- Dermatology
Cite this
Sensitivity of cross-reacting antihuman antibodies in formalin-fixed porcine skin : Including antibodies to proliferation antigens and cytokeratins with specificity in the skin. / Smith, Kathleen J.; Graham, John S.; Skelton, Henry G.; Hamilton, Tracey; O'Leary, Timothy; Okerberg, Carlin V.; Moeller, Robert; Hurst, Charles G.
In: Journal of Dermatological Science, Vol. 18, No. 1, 09.1998, p. 19-29.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sensitivity of cross-reacting antihuman antibodies in formalin-fixed porcine skin
T2 - Including antibodies to proliferation antigens and cytokeratins with specificity in the skin
AU - Smith, Kathleen J.
AU - Graham, John S.
AU - Skelton, Henry G.
AU - Hamilton, Tracey
AU - O'Leary, Timothy
AU - Okerberg, Carlin V.
AU - Moeller, Robert
AU - Hurst, Charles G.
PY - 1998/9
Y1 - 1998/9
N2 - Although no animal is a perfect skin model for the study of toxicological and therapeutic agents, structurally the pig may be superior to even non-human primates. Because our work involves effects of toxicological and therapeutic agents on the skin, we wanted to identify stains which may prove useful as well as determine cross-reactivity of some newer antihuman antibodies. We performed a battery of formalin-fixed skin from weanling pigs and minipigs. The battery of antibodies included LCA, CD3, OPD-4, CD34, UCHL- 1, L-26, KP-1, MAC-387, Factor XIIIa, Leu-7, S-100 protein, HMB-45, GFAP, synaptophysin, neurofilament protein, ubiquitin, vimentin, type IV collagen, laminin, fibronectin, Factor VIII related antigen, Desmin-M, smooth muscle actin, cytokeratin 7, cytokeratin 20, AE1/AE3, CAM 5.2, EMA, GCDFP, Ki-67, and PCNA. Immunohistochemical stains for CD3, Leu-7, S-100 protein, type IV collagen, laminin, Factor VIII related antigen, GFAP, synaptophysin, neurofilament protein, ubiquitin, smooth muscle actin, vimentin, Desmin-M, cytokeratin 7, cytokeratin 20, AE1/AE3, CAM 5.2, Ki-67 and PCNA showed consistent cross-reactivity. In formalin-fixed tissue, only antibodies to lymphoreticular cells showed poor cross-reactivity. A high percentage of the remaining antibodies did show good cross-reactivity but with some interesting similarities and differences in specificity.
AB - Although no animal is a perfect skin model for the study of toxicological and therapeutic agents, structurally the pig may be superior to even non-human primates. Because our work involves effects of toxicological and therapeutic agents on the skin, we wanted to identify stains which may prove useful as well as determine cross-reactivity of some newer antihuman antibodies. We performed a battery of formalin-fixed skin from weanling pigs and minipigs. The battery of antibodies included LCA, CD3, OPD-4, CD34, UCHL- 1, L-26, KP-1, MAC-387, Factor XIIIa, Leu-7, S-100 protein, HMB-45, GFAP, synaptophysin, neurofilament protein, ubiquitin, vimentin, type IV collagen, laminin, fibronectin, Factor VIII related antigen, Desmin-M, smooth muscle actin, cytokeratin 7, cytokeratin 20, AE1/AE3, CAM 5.2, EMA, GCDFP, Ki-67, and PCNA. Immunohistochemical stains for CD3, Leu-7, S-100 protein, type IV collagen, laminin, Factor VIII related antigen, GFAP, synaptophysin, neurofilament protein, ubiquitin, smooth muscle actin, vimentin, Desmin-M, cytokeratin 7, cytokeratin 20, AE1/AE3, CAM 5.2, Ki-67 and PCNA showed consistent cross-reactivity. In formalin-fixed tissue, only antibodies to lymphoreticular cells showed poor cross-reactivity. A high percentage of the remaining antibodies did show good cross-reactivity but with some interesting similarities and differences in specificity.
KW - Animal model
KW - Immunohistochemical stains
KW - Pig
UR - http://www.scopus.com/inward/record.url?scp=0031692260&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031692260&partnerID=8YFLogxK
U2 - 10.1016/S0923-1811(98)00018-8
DO - 10.1016/S0923-1811(98)00018-8
M3 - Article
C2 - 9747658
AN - SCOPUS:0031692260
VL - 18
SP - 19
EP - 29
JO - Journal of Dermatological Science
JF - Journal of Dermatological Science
SN - 0923-1811
IS - 1
ER -