Sensitivity of cross-reacting antihuman antibodies in formalin-fixed porcine skin: Including antibodies to proliferation antigens and cytokeratins with specificity in the skin

Kathleen J. Smith, John S. Graham, Henry G. Skelton, Tracey Hamilton, Timothy O'Leary, Carlin V. Okerberg, Robert Moeller, Charles G. Hurst

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Although no animal is a perfect skin model for the study of toxicological and therapeutic agents, structurally the pig may be superior to even non-human primates. Because our work involves effects of toxicological and therapeutic agents on the skin, we wanted to identify stains which may prove useful as well as determine cross-reactivity of some newer antihuman antibodies. We performed a battery of formalin-fixed skin from weanling pigs and minipigs. The battery of antibodies included LCA, CD3, OPD-4, CD34, UCHL- 1, L-26, KP-1, MAC-387, Factor XIIIa, Leu-7, S-100 protein, HMB-45, GFAP, synaptophysin, neurofilament protein, ubiquitin, vimentin, type IV collagen, laminin, fibronectin, Factor VIII related antigen, Desmin-M, smooth muscle actin, cytokeratin 7, cytokeratin 20, AE1/AE3, CAM 5.2, EMA, GCDFP, Ki-67, and PCNA. Immunohistochemical stains for CD3, Leu-7, S-100 protein, type IV collagen, laminin, Factor VIII related antigen, GFAP, synaptophysin, neurofilament protein, ubiquitin, smooth muscle actin, vimentin, Desmin-M, cytokeratin 7, cytokeratin 20, AE1/AE3, CAM 5.2, Ki-67 and PCNA showed consistent cross-reactivity. In formalin-fixed tissue, only antibodies to lymphoreticular cells showed poor cross-reactivity. A high percentage of the remaining antibodies did show good cross-reactivity but with some interesting similarities and differences in specificity.

Original languageEnglish (US)
Pages (from-to)19-29
Number of pages11
JournalJournal of Dermatological Science
Volume18
Issue number1
DOIs
StatePublished - Sep 1998
Externally publishedYes

Fingerprint

Keratins
Formaldehyde
Skin
Swine
Keratin-20
Keratin-7
Neurofilament Proteins
Antigens
Synaptophysin
Desmin
Collagen Type IV
S100 Proteins
Antibodies
Proliferating Cell Nuclear Antigen
von Willebrand Factor
Laminin
Vimentin
Computer aided manufacturing
Ubiquitin
Toxicology

Keywords

  • Animal model
  • Immunohistochemical stains
  • Pig

ASJC Scopus subject areas

  • Dermatology

Cite this

Sensitivity of cross-reacting antihuman antibodies in formalin-fixed porcine skin : Including antibodies to proliferation antigens and cytokeratins with specificity in the skin. / Smith, Kathleen J.; Graham, John S.; Skelton, Henry G.; Hamilton, Tracey; O'Leary, Timothy; Okerberg, Carlin V.; Moeller, Robert; Hurst, Charles G.

In: Journal of Dermatological Science, Vol. 18, No. 1, 09.1998, p. 19-29.

Research output: Contribution to journalArticle

Smith, Kathleen J. ; Graham, John S. ; Skelton, Henry G. ; Hamilton, Tracey ; O'Leary, Timothy ; Okerberg, Carlin V. ; Moeller, Robert ; Hurst, Charles G. / Sensitivity of cross-reacting antihuman antibodies in formalin-fixed porcine skin : Including antibodies to proliferation antigens and cytokeratins with specificity in the skin. In: Journal of Dermatological Science. 1998 ; Vol. 18, No. 1. pp. 19-29.
@article{52c20ef769f84ace8bcd9ee7c583a957,
title = "Sensitivity of cross-reacting antihuman antibodies in formalin-fixed porcine skin: Including antibodies to proliferation antigens and cytokeratins with specificity in the skin",
abstract = "Although no animal is a perfect skin model for the study of toxicological and therapeutic agents, structurally the pig may be superior to even non-human primates. Because our work involves effects of toxicological and therapeutic agents on the skin, we wanted to identify stains which may prove useful as well as determine cross-reactivity of some newer antihuman antibodies. We performed a battery of formalin-fixed skin from weanling pigs and minipigs. The battery of antibodies included LCA, CD3, OPD-4, CD34, UCHL- 1, L-26, KP-1, MAC-387, Factor XIIIa, Leu-7, S-100 protein, HMB-45, GFAP, synaptophysin, neurofilament protein, ubiquitin, vimentin, type IV collagen, laminin, fibronectin, Factor VIII related antigen, Desmin-M, smooth muscle actin, cytokeratin 7, cytokeratin 20, AE1/AE3, CAM 5.2, EMA, GCDFP, Ki-67, and PCNA. Immunohistochemical stains for CD3, Leu-7, S-100 protein, type IV collagen, laminin, Factor VIII related antigen, GFAP, synaptophysin, neurofilament protein, ubiquitin, smooth muscle actin, vimentin, Desmin-M, cytokeratin 7, cytokeratin 20, AE1/AE3, CAM 5.2, Ki-67 and PCNA showed consistent cross-reactivity. In formalin-fixed tissue, only antibodies to lymphoreticular cells showed poor cross-reactivity. A high percentage of the remaining antibodies did show good cross-reactivity but with some interesting similarities and differences in specificity.",
keywords = "Animal model, Immunohistochemical stains, Pig",
author = "Smith, {Kathleen J.} and Graham, {John S.} and Skelton, {Henry G.} and Tracey Hamilton and Timothy O'Leary and Okerberg, {Carlin V.} and Robert Moeller and Hurst, {Charles G.}",
year = "1998",
month = "9",
doi = "10.1016/S0923-1811(98)00018-8",
language = "English (US)",
volume = "18",
pages = "19--29",
journal = "Journal of Dermatological Science",
issn = "0923-1811",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Sensitivity of cross-reacting antihuman antibodies in formalin-fixed porcine skin

T2 - Including antibodies to proliferation antigens and cytokeratins with specificity in the skin

AU - Smith, Kathleen J.

AU - Graham, John S.

AU - Skelton, Henry G.

AU - Hamilton, Tracey

AU - O'Leary, Timothy

AU - Okerberg, Carlin V.

AU - Moeller, Robert

AU - Hurst, Charles G.

PY - 1998/9

Y1 - 1998/9

N2 - Although no animal is a perfect skin model for the study of toxicological and therapeutic agents, structurally the pig may be superior to even non-human primates. Because our work involves effects of toxicological and therapeutic agents on the skin, we wanted to identify stains which may prove useful as well as determine cross-reactivity of some newer antihuman antibodies. We performed a battery of formalin-fixed skin from weanling pigs and minipigs. The battery of antibodies included LCA, CD3, OPD-4, CD34, UCHL- 1, L-26, KP-1, MAC-387, Factor XIIIa, Leu-7, S-100 protein, HMB-45, GFAP, synaptophysin, neurofilament protein, ubiquitin, vimentin, type IV collagen, laminin, fibronectin, Factor VIII related antigen, Desmin-M, smooth muscle actin, cytokeratin 7, cytokeratin 20, AE1/AE3, CAM 5.2, EMA, GCDFP, Ki-67, and PCNA. Immunohistochemical stains for CD3, Leu-7, S-100 protein, type IV collagen, laminin, Factor VIII related antigen, GFAP, synaptophysin, neurofilament protein, ubiquitin, smooth muscle actin, vimentin, Desmin-M, cytokeratin 7, cytokeratin 20, AE1/AE3, CAM 5.2, Ki-67 and PCNA showed consistent cross-reactivity. In formalin-fixed tissue, only antibodies to lymphoreticular cells showed poor cross-reactivity. A high percentage of the remaining antibodies did show good cross-reactivity but with some interesting similarities and differences in specificity.

AB - Although no animal is a perfect skin model for the study of toxicological and therapeutic agents, structurally the pig may be superior to even non-human primates. Because our work involves effects of toxicological and therapeutic agents on the skin, we wanted to identify stains which may prove useful as well as determine cross-reactivity of some newer antihuman antibodies. We performed a battery of formalin-fixed skin from weanling pigs and minipigs. The battery of antibodies included LCA, CD3, OPD-4, CD34, UCHL- 1, L-26, KP-1, MAC-387, Factor XIIIa, Leu-7, S-100 protein, HMB-45, GFAP, synaptophysin, neurofilament protein, ubiquitin, vimentin, type IV collagen, laminin, fibronectin, Factor VIII related antigen, Desmin-M, smooth muscle actin, cytokeratin 7, cytokeratin 20, AE1/AE3, CAM 5.2, EMA, GCDFP, Ki-67, and PCNA. Immunohistochemical stains for CD3, Leu-7, S-100 protein, type IV collagen, laminin, Factor VIII related antigen, GFAP, synaptophysin, neurofilament protein, ubiquitin, smooth muscle actin, vimentin, Desmin-M, cytokeratin 7, cytokeratin 20, AE1/AE3, CAM 5.2, Ki-67 and PCNA showed consistent cross-reactivity. In formalin-fixed tissue, only antibodies to lymphoreticular cells showed poor cross-reactivity. A high percentage of the remaining antibodies did show good cross-reactivity but with some interesting similarities and differences in specificity.

KW - Animal model

KW - Immunohistochemical stains

KW - Pig

UR - http://www.scopus.com/inward/record.url?scp=0031692260&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031692260&partnerID=8YFLogxK

U2 - 10.1016/S0923-1811(98)00018-8

DO - 10.1016/S0923-1811(98)00018-8

M3 - Article

C2 - 9747658

AN - SCOPUS:0031692260

VL - 18

SP - 19

EP - 29

JO - Journal of Dermatological Science

JF - Journal of Dermatological Science

SN - 0923-1811

IS - 1

ER -