Sensitivity of cross-reacting antihuman antibodies in formalin-fixed porcine skin: Including antibodies to proliferation antigens and cytokeratins with specificity in the skin

Kathleen J. Smith, John S. Graham, Henry G. Skelton, Tracey Hamilton, Timothy O'Leary, Carlin V. Okerberg, Robert Moeller, Charles G. Hurst

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Although no animal is a perfect skin model for the study of toxicological and therapeutic agents, structurally the pig may be superior to even non-human primates. Because our work involves effects of toxicological and therapeutic agents on the skin, we wanted to identify stains which may prove useful as well as determine cross-reactivity of some newer antihuman antibodies. We performed a battery of formalin-fixed skin from weanling pigs and minipigs. The battery of antibodies included LCA, CD3, OPD-4, CD34, UCHL- 1, L-26, KP-1, MAC-387, Factor XIIIa, Leu-7, S-100 protein, HMB-45, GFAP, synaptophysin, neurofilament protein, ubiquitin, vimentin, type IV collagen, laminin, fibronectin, Factor VIII related antigen, Desmin-M, smooth muscle actin, cytokeratin 7, cytokeratin 20, AE1/AE3, CAM 5.2, EMA, GCDFP, Ki-67, and PCNA. Immunohistochemical stains for CD3, Leu-7, S-100 protein, type IV collagen, laminin, Factor VIII related antigen, GFAP, synaptophysin, neurofilament protein, ubiquitin, smooth muscle actin, vimentin, Desmin-M, cytokeratin 7, cytokeratin 20, AE1/AE3, CAM 5.2, Ki-67 and PCNA showed consistent cross-reactivity. In formalin-fixed tissue, only antibodies to lymphoreticular cells showed poor cross-reactivity. A high percentage of the remaining antibodies did show good cross-reactivity but with some interesting similarities and differences in specificity.

Original languageEnglish (US)
Pages (from-to)19-29
Number of pages11
JournalJournal of Dermatological Science
Volume18
Issue number1
DOIs
StatePublished - Sep 1998
Externally publishedYes

Keywords

  • Animal model
  • Immunohistochemical stains
  • Pig

ASJC Scopus subject areas

  • Dermatology

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