Abstract
Serum and fecal samples from a large California herd were used to estimate the diagnostic sensitivities and specificities of two ELISA kits and a fecal quantitative real time PCR (qPCR) for paratuberculosis. Both ELISA kits were marketed by the same company but one (Mycobacterium paratuberculosis; MP) was advertised as having increased sensitivity over an older kit that is no longer available (HerdChek; HC). Because of the large amount of data accumulated using the older kit, there was concern that the transition to a new test kit would provide significant complications in following herd level seroprevalence rates for Johne's disease. Furthermore, both kits diagnostic accuracy in large herds was not known. A 3 test (2 dependent, 1 independent) no gold standard analysis of the ELISA and qPCR results from a large dairy herd in California was conducted. Results of this study showed an improvement in the new ELISA kit compared to the previous one in terms of sensitivity (34% compared to 31%, respectively) and specificity (96% compared to 94%). The fecal qPCR assay had a sensitivity of 68% and a specificity of 96%. Prevalence of paratuberculosis in the study herd was 10.5%. Estimates reported here may differ from previous studies due to the differences in source herds, reference tests used and results variability due to laboratory and over time.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1-7 |
| Number of pages | 7 |
| Journal | International Journal of Applied Research in Veterinary Medicine |
| Volume | 12 |
| Issue number | 1 |
| State | Published - 2014 |
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Keywords
- ELISA
- Fecal qPCR
- Johne's
- Sensitivity and specificity
- Serum
ASJC Scopus subject areas
- veterinary(all)
Cite this
Sensitivity and specificity of two enzyme-linked immunosorbent assays and a quantitative real-time polymerase chain reaction for bovine paratuberculosis testing of a large dairy herd. / Aly, Sharif S; Anderson, R. J.; Whitlock, R. H.; Adaska, John M.
In: International Journal of Applied Research in Veterinary Medicine, Vol. 12, No. 1, 2014, p. 1-7.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Sensitivity and specificity of two enzyme-linked immunosorbent assays and a quantitative real-time polymerase chain reaction for bovine paratuberculosis testing of a large dairy herd
AU - Aly, Sharif S
AU - Anderson, R. J.
AU - Whitlock, R. H.
AU - Adaska, John M
PY - 2014
Y1 - 2014
N2 - Serum and fecal samples from a large California herd were used to estimate the diagnostic sensitivities and specificities of two ELISA kits and a fecal quantitative real time PCR (qPCR) for paratuberculosis. Both ELISA kits were marketed by the same company but one (Mycobacterium paratuberculosis; MP) was advertised as having increased sensitivity over an older kit that is no longer available (HerdChek; HC). Because of the large amount of data accumulated using the older kit, there was concern that the transition to a new test kit would provide significant complications in following herd level seroprevalence rates for Johne's disease. Furthermore, both kits diagnostic accuracy in large herds was not known. A 3 test (2 dependent, 1 independent) no gold standard analysis of the ELISA and qPCR results from a large dairy herd in California was conducted. Results of this study showed an improvement in the new ELISA kit compared to the previous one in terms of sensitivity (34% compared to 31%, respectively) and specificity (96% compared to 94%). The fecal qPCR assay had a sensitivity of 68% and a specificity of 96%. Prevalence of paratuberculosis in the study herd was 10.5%. Estimates reported here may differ from previous studies due to the differences in source herds, reference tests used and results variability due to laboratory and over time.
AB - Serum and fecal samples from a large California herd were used to estimate the diagnostic sensitivities and specificities of two ELISA kits and a fecal quantitative real time PCR (qPCR) for paratuberculosis. Both ELISA kits were marketed by the same company but one (Mycobacterium paratuberculosis; MP) was advertised as having increased sensitivity over an older kit that is no longer available (HerdChek; HC). Because of the large amount of data accumulated using the older kit, there was concern that the transition to a new test kit would provide significant complications in following herd level seroprevalence rates for Johne's disease. Furthermore, both kits diagnostic accuracy in large herds was not known. A 3 test (2 dependent, 1 independent) no gold standard analysis of the ELISA and qPCR results from a large dairy herd in California was conducted. Results of this study showed an improvement in the new ELISA kit compared to the previous one in terms of sensitivity (34% compared to 31%, respectively) and specificity (96% compared to 94%). The fecal qPCR assay had a sensitivity of 68% and a specificity of 96%. Prevalence of paratuberculosis in the study herd was 10.5%. Estimates reported here may differ from previous studies due to the differences in source herds, reference tests used and results variability due to laboratory and over time.
KW - ELISA
KW - Fecal qPCR
KW - Johne's
KW - Sensitivity and specificity
KW - Serum
UR - http://www.scopus.com/inward/record.url?scp=84900986666&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84900986666&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:84900986666
VL - 12
SP - 1
EP - 7
JO - International Journal of Applied Research in Veterinary Medicine
JF - International Journal of Applied Research in Veterinary Medicine
SN - 1542-2666
IS - 1
ER -