Sensitivity and specificity of two enzyme-linked immunosorbent assays and a quantitative real-time polymerase chain reaction for bovine paratuberculosis testing of a large dairy herd

Sharif S Aly, R. J. Anderson, R. H. Whitlock, John M Adaska

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Serum and fecal samples from a large California herd were used to estimate the diagnostic sensitivities and specificities of two ELISA kits and a fecal quantitative real time PCR (qPCR) for paratuberculosis. Both ELISA kits were marketed by the same company but one (Mycobacterium paratuberculosis; MP) was advertised as having increased sensitivity over an older kit that is no longer available (HerdChek; HC). Because of the large amount of data accumulated using the older kit, there was concern that the transition to a new test kit would provide significant complications in following herd level seroprevalence rates for Johne's disease. Furthermore, both kits diagnostic accuracy in large herds was not known. A 3 test (2 dependent, 1 independent) no gold standard analysis of the ELISA and qPCR results from a large dairy herd in California was conducted. Results of this study showed an improvement in the new ELISA kit compared to the previous one in terms of sensitivity (34% compared to 31%, respectively) and specificity (96% compared to 94%). The fecal qPCR assay had a sensitivity of 68% and a specificity of 96%. Prevalence of paratuberculosis in the study herd was 10.5%. Estimates reported here may differ from previous studies due to the differences in source herds, reference tests used and results variability due to laboratory and over time.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalInternational Journal of Applied Research in Veterinary Medicine
Volume12
Issue number1
StatePublished - 2014

Fingerprint

Paratuberculosis
paratuberculosis
dairy herds
Real-Time Polymerase Chain Reaction
quantitative polymerase chain reaction
Enzyme-Linked Immunosorbent Assay
herds
enzyme-linked immunosorbent assay
Sensitivity and Specificity
cattle
analytical kits
testing
Mycobacterium avium subsp. paratuberculosis
Seroepidemiologic Studies
diagnostic specificity
diagnostic sensitivity
seroprevalence
gold
Serum
assays

Keywords

  • ELISA
  • Fecal qPCR
  • Johne's
  • Sensitivity and specificity
  • Serum

ASJC Scopus subject areas

  • veterinary(all)

Cite this

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title = "Sensitivity and specificity of two enzyme-linked immunosorbent assays and a quantitative real-time polymerase chain reaction for bovine paratuberculosis testing of a large dairy herd",
abstract = "Serum and fecal samples from a large California herd were used to estimate the diagnostic sensitivities and specificities of two ELISA kits and a fecal quantitative real time PCR (qPCR) for paratuberculosis. Both ELISA kits were marketed by the same company but one (Mycobacterium paratuberculosis; MP) was advertised as having increased sensitivity over an older kit that is no longer available (HerdChek; HC). Because of the large amount of data accumulated using the older kit, there was concern that the transition to a new test kit would provide significant complications in following herd level seroprevalence rates for Johne's disease. Furthermore, both kits diagnostic accuracy in large herds was not known. A 3 test (2 dependent, 1 independent) no gold standard analysis of the ELISA and qPCR results from a large dairy herd in California was conducted. Results of this study showed an improvement in the new ELISA kit compared to the previous one in terms of sensitivity (34{\%} compared to 31{\%}, respectively) and specificity (96{\%} compared to 94{\%}). The fecal qPCR assay had a sensitivity of 68{\%} and a specificity of 96{\%}. Prevalence of paratuberculosis in the study herd was 10.5{\%}. Estimates reported here may differ from previous studies due to the differences in source herds, reference tests used and results variability due to laboratory and over time.",
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AU - Adaska, John M

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