Sensitive and specific identification of Neospora caninum infection of cattle based on detection of serum antibodies to recombinant Ncp29

Daniel K. Howe, Keliang Tang, Patricia A Conrad, Karen Sverlow, J. P. Dubey, L. David Sibley

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Neosporosis is an economically important disease of dairy cattle caused by the protozoan Neospora caninum. Diagnostic tests for neosporosis are complicated by the potential for cross-reaction of antibodies to antigens that are similar between N. caninum and closely related parasites Toxoplasma gondii and Sarcocystis cruzi. To provide a sensitive and specific assay for detecting antibodies to N. caninum in the serum of infected animals, we have investigated a recombinant form of the antigen known as Ncp29 (rNcp29), which is a major surface protein of the parasite. Ncp29 is encoded by a gene that is homologous to the SAG1 gene previously characterized from T. gondii. An enzyme-linked immunosorbent assay (ELISA) was used to screen animals for the presence of serum antibodies specific to rNcp29. The rNcp29 ELISA readily distinguished between cattle known to be infected with N. caninum (optical density [OD] > 1.2 at 1:500 or greater dilution) and negative controls (OD < 0.5 at 1:500). Additionally, sera from animals that were infected with T. gondii or S. cruzi were negative. The rNcp29 ELISA developed here provides a specific and sensitive assay for detecting neosporosis in cattle.

Original languageEnglish (US)
Pages (from-to)611-615
Number of pages5
JournalClinical and Diagnostic Laboratory Immunology
Volume9
Issue number3
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Neospora
Assays
Toxoplasma
Immunosorbents
Antibodies
Enzyme-Linked Immunosorbent Assay
Infection
Serum
Animals
Density (optical)
Parasites
Sarcocystis
Cattle Diseases
Antigens
Enzymes
Genes
Cross Reactions
Routine Diagnostic Tests
Dairies
Membrane Proteins

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology and Allergy
  • Clinical Biochemistry
  • Immunology

Cite this

Sensitive and specific identification of Neospora caninum infection of cattle based on detection of serum antibodies to recombinant Ncp29. / Howe, Daniel K.; Tang, Keliang; Conrad, Patricia A; Sverlow, Karen; Dubey, J. P.; Sibley, L. David.

In: Clinical and Diagnostic Laboratory Immunology, Vol. 9, No. 3, 2002, p. 611-615.

Research output: Contribution to journalArticle

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