Semicontinuous flow centrifugation for the pheresis of immunocompetent cells and stem cells

R. S. Weiner, Carol M Richman, R. A. Yankee

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Mononuclear cells from human peripheral blood were purified by semicontinuous flow centrifugation (SCFC) using the Haemonetics model 30 blood cell separator; 64% ± 7% of the mononuclear cells in 600 ml of peripheral blood were collected in a 30 ml volume. Analysis of sequential 5 ml aliquots of the mononuclear cell concentrate revealed that both immunocompetent cells and granulocytic progenitor cells (CFU C) were proportional to the cell count throughout the buffy coat. In vitro pheresis of large volumes of human bone marrow resulted in recovery pf 63% of the cells, 12% of the hemoglobin, and 84% of the CFU C in 20% of the original volume. Further centrifugation eliminated 80% of the platelets without loss of cells or CFU C. SCFC of peripheral blood of bone marrow selectively concentrated mononuclear cells and reduced the contamination by granulocytes and erythrocytes. Large numbers of mononuclear cells can thus be collected for studies in vitro or for cryopreservation and the autologous reconstitution of immunosuppressed or myelosuppressed patients undergoing intensive antitumor therapy.

Original languageEnglish (US)
Pages (from-to)391-397
Number of pages7
JournalBlood
Volume49
Issue number3
StatePublished - 1977
Externally publishedYes

Fingerprint

Blood Component Removal
Centrifugation
Stem cells
Blood
Stem Cells
Bone
Platelets
Separators
Cell Count
Bone Marrow
Hemoglobins
Contamination
Cells
Recovery
Cryopreservation
Granulocytes
Blood Cells
Blood Platelets
Erythrocytes

ASJC Scopus subject areas

  • Hematology

Cite this

Semicontinuous flow centrifugation for the pheresis of immunocompetent cells and stem cells. / Weiner, R. S.; Richman, Carol M; Yankee, R. A.

In: Blood, Vol. 49, No. 3, 1977, p. 391-397.

Research output: Contribution to journalArticle

@article{e41fed87f1094a179cb0b06b3a4966d7,
title = "Semicontinuous flow centrifugation for the pheresis of immunocompetent cells and stem cells",
abstract = "Mononuclear cells from human peripheral blood were purified by semicontinuous flow centrifugation (SCFC) using the Haemonetics model 30 blood cell separator; 64{\%} ± 7{\%} of the mononuclear cells in 600 ml of peripheral blood were collected in a 30 ml volume. Analysis of sequential 5 ml aliquots of the mononuclear cell concentrate revealed that both immunocompetent cells and granulocytic progenitor cells (CFU C) were proportional to the cell count throughout the buffy coat. In vitro pheresis of large volumes of human bone marrow resulted in recovery pf 63{\%} of the cells, 12{\%} of the hemoglobin, and 84{\%} of the CFU C in 20{\%} of the original volume. Further centrifugation eliminated 80{\%} of the platelets without loss of cells or CFU C. SCFC of peripheral blood of bone marrow selectively concentrated mononuclear cells and reduced the contamination by granulocytes and erythrocytes. Large numbers of mononuclear cells can thus be collected for studies in vitro or for cryopreservation and the autologous reconstitution of immunosuppressed or myelosuppressed patients undergoing intensive antitumor therapy.",
author = "Weiner, {R. S.} and Richman, {Carol M} and Yankee, {R. A.}",
year = "1977",
language = "English (US)",
volume = "49",
pages = "391--397",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "3",

}

TY - JOUR

T1 - Semicontinuous flow centrifugation for the pheresis of immunocompetent cells and stem cells

AU - Weiner, R. S.

AU - Richman, Carol M

AU - Yankee, R. A.

PY - 1977

Y1 - 1977

N2 - Mononuclear cells from human peripheral blood were purified by semicontinuous flow centrifugation (SCFC) using the Haemonetics model 30 blood cell separator; 64% ± 7% of the mononuclear cells in 600 ml of peripheral blood were collected in a 30 ml volume. Analysis of sequential 5 ml aliquots of the mononuclear cell concentrate revealed that both immunocompetent cells and granulocytic progenitor cells (CFU C) were proportional to the cell count throughout the buffy coat. In vitro pheresis of large volumes of human bone marrow resulted in recovery pf 63% of the cells, 12% of the hemoglobin, and 84% of the CFU C in 20% of the original volume. Further centrifugation eliminated 80% of the platelets without loss of cells or CFU C. SCFC of peripheral blood of bone marrow selectively concentrated mononuclear cells and reduced the contamination by granulocytes and erythrocytes. Large numbers of mononuclear cells can thus be collected for studies in vitro or for cryopreservation and the autologous reconstitution of immunosuppressed or myelosuppressed patients undergoing intensive antitumor therapy.

AB - Mononuclear cells from human peripheral blood were purified by semicontinuous flow centrifugation (SCFC) using the Haemonetics model 30 blood cell separator; 64% ± 7% of the mononuclear cells in 600 ml of peripheral blood were collected in a 30 ml volume. Analysis of sequential 5 ml aliquots of the mononuclear cell concentrate revealed that both immunocompetent cells and granulocytic progenitor cells (CFU C) were proportional to the cell count throughout the buffy coat. In vitro pheresis of large volumes of human bone marrow resulted in recovery pf 63% of the cells, 12% of the hemoglobin, and 84% of the CFU C in 20% of the original volume. Further centrifugation eliminated 80% of the platelets without loss of cells or CFU C. SCFC of peripheral blood of bone marrow selectively concentrated mononuclear cells and reduced the contamination by granulocytes and erythrocytes. Large numbers of mononuclear cells can thus be collected for studies in vitro or for cryopreservation and the autologous reconstitution of immunosuppressed or myelosuppressed patients undergoing intensive antitumor therapy.

UR - http://www.scopus.com/inward/record.url?scp=0017364134&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017364134&partnerID=8YFLogxK

M3 - Article

C2 - 138455

AN - SCOPUS:0017364134

VL - 49

SP - 391

EP - 397

JO - Blood

JF - Blood

SN - 0006-4971

IS - 3

ER -