Abstract
Semaphorin signaling plays integral roles in multiple developmental processes. Branching morphogenesis is one such role that has not been thoroughly explored. Here, we show in mice that functional blockage of neuropilin 1 (Npn1) inhibits cleft formation in the developing submandibular gland (SMG) cultured ex vivo. This Npn1-dependent morphogenesis is mediated by Sema3A and Sema3C in an additive manner, and can be abolished by decreasing the expression of plexin A2 or plexin D1. VEGF, another known Npn1 ligand, has no apparent effects on SMG development. FGF signaling, which also mediates SMG branching morphogenesis, acts in parallel with semaphorin signaling. Finally, in contrast to the effect of FGF signaling, we find that semaphorins do not stimulate the proliferation of SMG epithelial cells. Instead, the semaphorin signals act locally on the epithelial cells to facilitate SMG cleft formation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2935-2945 |
| Number of pages | 11 |
| Journal | Development |
| Volume | 134 |
| Issue number | 16 |
| DOIs | |
| State | Published - Aug 2007 |
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Keywords
- Branching morphogenesis
- Class 3 semaphorin
- Cleft formation
- Mouse
- Neuropilin (Npn; Nrp1)
- Plexin
- Salivary gland
ASJC Scopus subject areas
- Anatomy
- Cell Biology
Cite this
Semaphorin signaling facilitates cleft formation in the developing salivary gland. / Chung, Ling; Yang, Tsung Lin; Huang, Hsiu Ru; Hsu, Su Ming; Cheng, Hwai-Jong; Huang, Pei Hsin.
In: Development, Vol. 134, No. 16, 08.2007, p. 2935-2945.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Semaphorin signaling facilitates cleft formation in the developing salivary gland
AU - Chung, Ling
AU - Yang, Tsung Lin
AU - Huang, Hsiu Ru
AU - Hsu, Su Ming
AU - Cheng, Hwai-Jong
AU - Huang, Pei Hsin
PY - 2007/8
Y1 - 2007/8
N2 - Semaphorin signaling plays integral roles in multiple developmental processes. Branching morphogenesis is one such role that has not been thoroughly explored. Here, we show in mice that functional blockage of neuropilin 1 (Npn1) inhibits cleft formation in the developing submandibular gland (SMG) cultured ex vivo. This Npn1-dependent morphogenesis is mediated by Sema3A and Sema3C in an additive manner, and can be abolished by decreasing the expression of plexin A2 or plexin D1. VEGF, another known Npn1 ligand, has no apparent effects on SMG development. FGF signaling, which also mediates SMG branching morphogenesis, acts in parallel with semaphorin signaling. Finally, in contrast to the effect of FGF signaling, we find that semaphorins do not stimulate the proliferation of SMG epithelial cells. Instead, the semaphorin signals act locally on the epithelial cells to facilitate SMG cleft formation.
AB - Semaphorin signaling plays integral roles in multiple developmental processes. Branching morphogenesis is one such role that has not been thoroughly explored. Here, we show in mice that functional blockage of neuropilin 1 (Npn1) inhibits cleft formation in the developing submandibular gland (SMG) cultured ex vivo. This Npn1-dependent morphogenesis is mediated by Sema3A and Sema3C in an additive manner, and can be abolished by decreasing the expression of plexin A2 or plexin D1. VEGF, another known Npn1 ligand, has no apparent effects on SMG development. FGF signaling, which also mediates SMG branching morphogenesis, acts in parallel with semaphorin signaling. Finally, in contrast to the effect of FGF signaling, we find that semaphorins do not stimulate the proliferation of SMG epithelial cells. Instead, the semaphorin signals act locally on the epithelial cells to facilitate SMG cleft formation.
KW - Branching morphogenesis
KW - Class 3 semaphorin
KW - Cleft formation
KW - Mouse
KW - Neuropilin (Npn; Nrp1)
KW - Plexin
KW - Salivary gland
UR - http://www.scopus.com/inward/record.url?scp=34548546568&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34548546568&partnerID=8YFLogxK
U2 - 10.1242/dev.005066
DO - 10.1242/dev.005066
M3 - Article
C2 - 17626059
AN - SCOPUS:34548546568
VL - 134
SP - 2935
EP - 2945
JO - Development (Cambridge)
JF - Development (Cambridge)
SN - 0950-1991
IS - 16
ER -