Semaphorin 6C leads to GSK-3-dependent growth cone collapse and redistributes after entorhino-hippocampal axotomy

Ferran Burgaya, Xavier Fontana, Albert Martínez, Marisol Montolio, Ana Mingorance, Sergi Simo Olivar, José Antonio del Río, Eduardo Soriano

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


We studied the changes in the distribution of a specific variant of Semaphorin Y/6C (Sema6C) in mouse forebrain after axotomy of the entorhino-hippocampal perforant pathway. We found this isoform to be widely expressed during development, remaining in the adult and showing variations in distribution when the perforant pathway was axotomized. These changes were detected in both the hippocampal and entorhinal cortices. Sema6C1 immunoreactivity (IR) was high in the stratum radiatum of the hippocampus proper and the inner molecular layer of the dentate gyrus; the entorhinal cortex showed Sema6C1 IR in both cell bodies and in fibers of the II/III and V/VI layers. In axotomized animals, the IR of the ipsilateral, but not the contralateral, hemisphere showed that IR had moved into the stratum lacunosum-moleculare, the medial molecular layer of the dentate gyrus and the fibers, but not the cell bodies, of the entorhinal cortex. These results were not reproduced after lateral axotomy of the fimbria fornix, indicating a specific role for Sema6C variants in the generation and/or stability of entorhino-hippocampal synapses. Growth cone collapse of entorhinal and pyramidal neurons, as well as activation of glycogen synthase kinase-3 (GSK-3) through depletion of the inactive pool, induced by diffusible Sema6C1 further supports this view.

Original languageEnglish (US)
Pages (from-to)321-334
Number of pages14
JournalMolecular and Cellular Neuroscience
Issue number3
StatePublished - Nov 6 2006
Externally publishedYes


  • Axon Guidance
  • Axotomy
  • Hippocampal formation
  • Perforant
  • Plasticity
  • Post-synaptic
  • Pyramidal cell
  • Semaphorin
  • Synaptogenesis

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology


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