Self-organization of engineered epithelial tubules by differential cellular motility

Hidetoshi Mori, Nikolce Gjorevski, Jamie L. Inman, Mina J. Bissell, Celeste M. Nelson

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Patterning of developing tissues arises from a number of mechanisms, including cell shape change, cell proliferation, and cell sorting from differential cohesion or tension. Here,wereveal that differences in cell motility can also lead to cell sorting within tissues. Using mosaic engineered mammary epithelial tubules, we found that cells sorted depending on their expression level of the membrane-anchored collagenase matrix metalloproteinase (MMP)-14. These rearrangements were independent of the catalytic activity of MMP14 but absolutely required the hemopexin domain. We describe a signaling cascade downstream of MMP14 through Rho kinase that allows cells to sort within the model tissues. Cell speed and persistence time were enhanced by MMP14 expression, but only the latter motility parameter was required for sorting. These results indicate that differential directional persistence can give rise to patterns within model developing tissues.

Original languageEnglish (US)
Pages (from-to)14890-14895
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number35
DOIs
StatePublished - Sep 1 2009
Externally publishedYes

Fingerprint

Hemopexin
Matrix Metalloproteinase 14
rho-Associated Kinases
Cell Shape
Collagenases
Cell Movement
Breast
Cell Proliferation
Membranes

Keywords

  • Differential adhesion
  • Micropatterning
  • Morphogenesis
  • MT1-MMP
  • Tissue patterning

ASJC Scopus subject areas

  • General

Cite this

Self-organization of engineered epithelial tubules by differential cellular motility. / Mori, Hidetoshi; Gjorevski, Nikolce; Inman, Jamie L.; Bissell, Mina J.; Nelson, Celeste M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, No. 35, 01.09.2009, p. 14890-14895.

Research output: Contribution to journalArticle

Mori, Hidetoshi ; Gjorevski, Nikolce ; Inman, Jamie L. ; Bissell, Mina J. ; Nelson, Celeste M. / Self-organization of engineered epithelial tubules by differential cellular motility. In: Proceedings of the National Academy of Sciences of the United States of America. 2009 ; Vol. 106, No. 35. pp. 14890-14895.
@article{3968d885e5304c219360997b785de7f9,
title = "Self-organization of engineered epithelial tubules by differential cellular motility",
abstract = "Patterning of developing tissues arises from a number of mechanisms, including cell shape change, cell proliferation, and cell sorting from differential cohesion or tension. Here,wereveal that differences in cell motility can also lead to cell sorting within tissues. Using mosaic engineered mammary epithelial tubules, we found that cells sorted depending on their expression level of the membrane-anchored collagenase matrix metalloproteinase (MMP)-14. These rearrangements were independent of the catalytic activity of MMP14 but absolutely required the hemopexin domain. We describe a signaling cascade downstream of MMP14 through Rho kinase that allows cells to sort within the model tissues. Cell speed and persistence time were enhanced by MMP14 expression, but only the latter motility parameter was required for sorting. These results indicate that differential directional persistence can give rise to patterns within model developing tissues.",
keywords = "Differential adhesion, Micropatterning, Morphogenesis, MT1-MMP, Tissue patterning",
author = "Hidetoshi Mori and Nikolce Gjorevski and Inman, {Jamie L.} and Bissell, {Mina J.} and Nelson, {Celeste M.}",
year = "2009",
month = "9",
day = "1",
doi = "10.1073/pnas.0901269106",
language = "English (US)",
volume = "106",
pages = "14890--14895",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "35",

}

TY - JOUR

T1 - Self-organization of engineered epithelial tubules by differential cellular motility

AU - Mori, Hidetoshi

AU - Gjorevski, Nikolce

AU - Inman, Jamie L.

AU - Bissell, Mina J.

AU - Nelson, Celeste M.

PY - 2009/9/1

Y1 - 2009/9/1

N2 - Patterning of developing tissues arises from a number of mechanisms, including cell shape change, cell proliferation, and cell sorting from differential cohesion or tension. Here,wereveal that differences in cell motility can also lead to cell sorting within tissues. Using mosaic engineered mammary epithelial tubules, we found that cells sorted depending on their expression level of the membrane-anchored collagenase matrix metalloproteinase (MMP)-14. These rearrangements were independent of the catalytic activity of MMP14 but absolutely required the hemopexin domain. We describe a signaling cascade downstream of MMP14 through Rho kinase that allows cells to sort within the model tissues. Cell speed and persistence time were enhanced by MMP14 expression, but only the latter motility parameter was required for sorting. These results indicate that differential directional persistence can give rise to patterns within model developing tissues.

AB - Patterning of developing tissues arises from a number of mechanisms, including cell shape change, cell proliferation, and cell sorting from differential cohesion or tension. Here,wereveal that differences in cell motility can also lead to cell sorting within tissues. Using mosaic engineered mammary epithelial tubules, we found that cells sorted depending on their expression level of the membrane-anchored collagenase matrix metalloproteinase (MMP)-14. These rearrangements were independent of the catalytic activity of MMP14 but absolutely required the hemopexin domain. We describe a signaling cascade downstream of MMP14 through Rho kinase that allows cells to sort within the model tissues. Cell speed and persistence time were enhanced by MMP14 expression, but only the latter motility parameter was required for sorting. These results indicate that differential directional persistence can give rise to patterns within model developing tissues.

KW - Differential adhesion

KW - Micropatterning

KW - Morphogenesis

KW - MT1-MMP

KW - Tissue patterning

UR - http://www.scopus.com/inward/record.url?scp=70349283085&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349283085&partnerID=8YFLogxK

U2 - 10.1073/pnas.0901269106

DO - 10.1073/pnas.0901269106

M3 - Article

C2 - 19706461

AN - SCOPUS:70349283085

VL - 106

SP - 14890

EP - 14895

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 35

ER -