Abstract
Mechanisms underlying the organization of centrosome-derived microtubule arrays are well understood, but less is known about how acentrosomal microtubule networks are formed. The basal cortex of polarized epithelial cells contains a microtubule network of mixed polarity. We examined how this network is organized by imaging microtubule dynamics in acentrosomal basal cytoplasts derived from these cells. We show that the steady-state microtubule network appears to form by a combination of microtubule-microtubule and microtubule-cortex interactions, both of which increase microtubule stability. We used computational modeling to determine whether these microtubule parameters are sufficient to generate a steady-state acentrosomal microtubule network. Microtubules undergoing dynamic instability without any stabilization points continuously remodel their organization without reaching a steady-state network. However, the addition of increased microtubule stabilization at microtubule-microtubule and microtubule-cortex interactions results in the rapid assembly of a steady-state microtubule network in silico that is remarkably similar to networks formed in situ. These results define minimal parameters for the self-organization of an acentrosomal microtubule network.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 845-855 |
| Number of pages | 11 |
| Journal | Journal of Cell Biology |
| Volume | 171 |
| Issue number | 5 |
| DOIs | |
| State | Published - Dec 5 2005 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cell Biology
Cite this
Self-organization of an acentrosomal microtubule network at the basal cortex of polarized epithelial cells. / Reilein, Amy; Yamada, Soichiro; Nelson, W. James.
In: Journal of Cell Biology, Vol. 171, No. 5, 05.12.2005, p. 845-855.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Self-organization of an acentrosomal microtubule network at the basal cortex of polarized epithelial cells
AU - Reilein, Amy
AU - Yamada, Soichiro
AU - Nelson, W. James
PY - 2005/12/5
Y1 - 2005/12/5
N2 - Mechanisms underlying the organization of centrosome-derived microtubule arrays are well understood, but less is known about how acentrosomal microtubule networks are formed. The basal cortex of polarized epithelial cells contains a microtubule network of mixed polarity. We examined how this network is organized by imaging microtubule dynamics in acentrosomal basal cytoplasts derived from these cells. We show that the steady-state microtubule network appears to form by a combination of microtubule-microtubule and microtubule-cortex interactions, both of which increase microtubule stability. We used computational modeling to determine whether these microtubule parameters are sufficient to generate a steady-state acentrosomal microtubule network. Microtubules undergoing dynamic instability without any stabilization points continuously remodel their organization without reaching a steady-state network. However, the addition of increased microtubule stabilization at microtubule-microtubule and microtubule-cortex interactions results in the rapid assembly of a steady-state microtubule network in silico that is remarkably similar to networks formed in situ. These results define minimal parameters for the self-organization of an acentrosomal microtubule network.
AB - Mechanisms underlying the organization of centrosome-derived microtubule arrays are well understood, but less is known about how acentrosomal microtubule networks are formed. The basal cortex of polarized epithelial cells contains a microtubule network of mixed polarity. We examined how this network is organized by imaging microtubule dynamics in acentrosomal basal cytoplasts derived from these cells. We show that the steady-state microtubule network appears to form by a combination of microtubule-microtubule and microtubule-cortex interactions, both of which increase microtubule stability. We used computational modeling to determine whether these microtubule parameters are sufficient to generate a steady-state acentrosomal microtubule network. Microtubules undergoing dynamic instability without any stabilization points continuously remodel their organization without reaching a steady-state network. However, the addition of increased microtubule stabilization at microtubule-microtubule and microtubule-cortex interactions results in the rapid assembly of a steady-state microtubule network in silico that is remarkably similar to networks formed in situ. These results define minimal parameters for the self-organization of an acentrosomal microtubule network.
UR - http://www.scopus.com/inward/record.url?scp=28544449479&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=28544449479&partnerID=8YFLogxK
U2 - 10.1083/jcb.200505071
DO - 10.1083/jcb.200505071
M3 - Article
C2 - 16314429
AN - SCOPUS:28544449479
VL - 171
SP - 845
EP - 855
JO - Journal of Cell Biology
JF - Journal of Cell Biology
SN - 0021-9525
IS - 5
ER -