Self-assembly of fibrochondrocytes and chondrocytes for tissue engineering of the knee meniscus

Gwendolyn M. Hoben, Jerry C. Hu, Regis A. James, Kyriacos A. Athanasiou

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Chondrocyte self-assembly in high-density scaffoldless culture has shown success in producing articular cartilage constructs, and a similar process could be applied to fibrocartilage tissue engineering. Three cell combinations were compared in self-assembly culture - 100% chondrocytes, 100% meniscal fibrochondrocytes, and 50:50 co-cultures of fibrochondrocytes and chondrocytes with the goal of creating a proteoglycan, collagen I, and collagen II matrix similar to native meniscus. Two culture surfaces were also compared for self-assembly: agarose-coated wells and tissue culture plastic. After 4 weeks, the resulting self-assembled chondrocyte constructs were 10.24 ± 0.63 mm in diameter and 0.96 ± 0.14 mm thick, weighing 84.5 ± 7.2 mg. Co-culture constructs were smaller and weighed 22.5 ± 1.0 mg. In contrast, the fibrochondrocyte constructs contracted into spheres weighing 1.3 ± 0.3 mg. Immunostaining showed collagen II in the chondrocyte constructs, both collagen I and collagen II in the co-cultures, and only collagen I in the fibrochondrocyte constructs. Collagen densities for chondrocyte, co-culture, and fibrochondrocyte constructs were 41 ± 3, 38 ± 3, and 20 ± 2 μg/mg dry weight, and glycosaminoglycan densities were 230 ± 2, 80 ± 6, and 10 ± 1 μg/mg dry weight, respectively. Self-assembled co-cultures, with their mixed collagen I and II matrix and robust gross characteristics, appear promising for tissue engineering of the knee meniscus.

Original languageEnglish (US)
Pages (from-to)939-946
Number of pages8
JournalTissue Engineering
Volume13
Issue number5
DOIs
StatePublished - May 2007
Externally publishedYes

Fingerprint

Tissue Engineering
Chondrocytes
Tissue engineering
Collagen
Self assembly
Knee
Coculture Techniques
Weighing
Fibrocartilage
Weights and Measures
Tissue culture
Meniscus
Cartilage
Articular Cartilage
Proteoglycans
Glycosaminoglycans
Sepharose
Plastics

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Biotechnology

Cite this

Self-assembly of fibrochondrocytes and chondrocytes for tissue engineering of the knee meniscus. / Hoben, Gwendolyn M.; Hu, Jerry C.; James, Regis A.; Athanasiou, Kyriacos A.

In: Tissue Engineering, Vol. 13, No. 5, 05.2007, p. 939-946.

Research output: Contribution to journalArticle

Hoben, Gwendolyn M. ; Hu, Jerry C. ; James, Regis A. ; Athanasiou, Kyriacos A. / Self-assembly of fibrochondrocytes and chondrocytes for tissue engineering of the knee meniscus. In: Tissue Engineering. 2007 ; Vol. 13, No. 5. pp. 939-946.
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