Selenium functionalized intraocular lenses inhibit posterior capsule opacification in an ex vivo canine lens capsular bag assay

Simon A. Pot, Heather L. Chandler, Carmen M H Colitz, Ellison Bentley, Richard R. Dubielzig, Thomas S. Mosley, Ted W. Reid, Christopher J Murphy

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The purpose of this study was to determine the inhibitory effect of selenocystamine coated intraocular lenses (IOLs) on the formation of posterior capsule opacification (PCO) in an ex vivo canine lens capsular bag assay. Selenocystamine was covalently bound to the surface of poly(2-hydroxyethyl methacrylate) (poly(HEMA)) discs. Three groups of canine lens capsules (6 coated IOLs (SeIOLs), 7 non-coated control IOLs and 8 empty capsules) were cultured for 10 days. During the culture period PCO was scored based on visual inspection of the capsules using phase contrast microscopy. On day 10 all the capsules were prepared for light microscopic examination and lens epithelial cells (LECs) were quantified. Proliferating cell nuclear antigen (PCNA), α-smooth muscle actin (α-SMA) and cleaved caspase-3 were examined by immunohistochemistry. Additionally, cell viability assays were performed on LECs cultured in tissue culture medium pre-incubated with either a SeIOL or control IOL. The viability assays demonstrated that no detectable cytotoxic leachables were associated with the functionalized IOLs. The central posterior capsule was free of cells underneath all SeIOLs, although large numbers of LECs populated the capsular periphery. Apoptotic cells were observed underneath the periphery of some SeIOLs. Both the PCO scores and LEC counts of SeIOL containing capsules were significantly lower than those of control group capsules (p < 0.01 and p = 0.0004, respectively). The use of selenium functionalized IOLs resulted in a significant reduction of PCO in this ex vivo model. Binding of selenocystamine to a foldable IOL may provide an effective method to prevent population of the central posterior capsule with LECs.

Original languageEnglish (US)
Pages (from-to)728-734
Number of pages7
JournalExperimental Eye Research
Volume89
Issue number5
DOIs
StatePublished - Nov 2009
Externally publishedYes

Fingerprint

Posterior Capsule of the Lens
Capsule Opacification
Intraocular Lenses
Selenium
Lenses
Capsules
Canidae
Epithelial Cells
Phase-Contrast Microscopy
Proliferating Cell Nuclear Antigen
Caspase 3
Smooth Muscle
Culture Media
Actins
Cell Survival
Cell Count
Immunohistochemistry
Light
Control Groups

Keywords

  • apoptosis
  • cell culture
  • intraocular lens
  • lens capsule
  • posterior capsule opacification
  • selenium

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Selenium functionalized intraocular lenses inhibit posterior capsule opacification in an ex vivo canine lens capsular bag assay. / Pot, Simon A.; Chandler, Heather L.; Colitz, Carmen M H; Bentley, Ellison; Dubielzig, Richard R.; Mosley, Thomas S.; Reid, Ted W.; Murphy, Christopher J.

In: Experimental Eye Research, Vol. 89, No. 5, 11.2009, p. 728-734.

Research output: Contribution to journalArticle

Pot, Simon A. ; Chandler, Heather L. ; Colitz, Carmen M H ; Bentley, Ellison ; Dubielzig, Richard R. ; Mosley, Thomas S. ; Reid, Ted W. ; Murphy, Christopher J. / Selenium functionalized intraocular lenses inhibit posterior capsule opacification in an ex vivo canine lens capsular bag assay. In: Experimental Eye Research. 2009 ; Vol. 89, No. 5. pp. 728-734.
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abstract = "The purpose of this study was to determine the inhibitory effect of selenocystamine coated intraocular lenses (IOLs) on the formation of posterior capsule opacification (PCO) in an ex vivo canine lens capsular bag assay. Selenocystamine was covalently bound to the surface of poly(2-hydroxyethyl methacrylate) (poly(HEMA)) discs. Three groups of canine lens capsules (6 coated IOLs (SeIOLs), 7 non-coated control IOLs and 8 empty capsules) were cultured for 10 days. During the culture period PCO was scored based on visual inspection of the capsules using phase contrast microscopy. On day 10 all the capsules were prepared for light microscopic examination and lens epithelial cells (LECs) were quantified. Proliferating cell nuclear antigen (PCNA), α-smooth muscle actin (α-SMA) and cleaved caspase-3 were examined by immunohistochemistry. Additionally, cell viability assays were performed on LECs cultured in tissue culture medium pre-incubated with either a SeIOL or control IOL. The viability assays demonstrated that no detectable cytotoxic leachables were associated with the functionalized IOLs. The central posterior capsule was free of cells underneath all SeIOLs, although large numbers of LECs populated the capsular periphery. Apoptotic cells were observed underneath the periphery of some SeIOLs. Both the PCO scores and LEC counts of SeIOL containing capsules were significantly lower than those of control group capsules (p < 0.01 and p = 0.0004, respectively). The use of selenium functionalized IOLs resulted in a significant reduction of PCO in this ex vivo model. Binding of selenocystamine to a foldable IOL may provide an effective method to prevent population of the central posterior capsule with LECs.",
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AU - Mosley, Thomas S.

AU - Reid, Ted W.

AU - Murphy, Christopher J

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