Selectively enhanced contextual fear conditioning in mice lacking the transcriptional regulator CCAAT/enhancer binding protein δ

Esta Sterneck, Richard Paylor, Vernice Jackson-Lewis, Megan Libbey, Serge Przedborski, Lino Tessarollo, Jacqueline Crawley, Peter F. Johnson

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

CCAAT/enhancer binding protein δ (C/EBPδ) is a transcriptional regulator implicated in the hepatic acute phase response and in adipogenic and myeloid cell differentiation. We found that C/EBPδ is widely expressed in the peripheral and central nervous systems, including neurons of the hippocampal formation, indicating a role in neural functions. To examine the role of C/EBPδ in vivo, we generated mice with a targeted deletion of the C/EBPδ gene. This mutation does not interfere with normal embryonic and postnatal development. Performance in a battery of behavioral tests indicates that basic neurological functions are normal. Furthermore, performance in a Morris water maze task suggests that C/EBPδ mutant mice have normal spatial learning. However, in the contextual and auditory-cue-conditioned fear task, C/EBPδ null mice displayed significantly more conditioned freezing to the test context than did wild-type controls, but equivalent conditioning to the auditory cue. These data demonstrate a selectively enhanced contextual fear response in mice carrying a targeted genomic mutation and implicate C/EBPδ in the regulation of a specific type of learning and memory.

Original languageEnglish (US)
Pages (from-to)10908-10913
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number18
DOIs
StatePublished - Sep 1 1998
Externally publishedYes

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CCAAT-Enhancer-Binding Proteins
Fear
Cues
Mutation
Acute-Phase Reaction
Peripheral Nervous System
Myeloid Cells
Conditioning (Psychology)
Freezing
Embryonic Development
Cell Differentiation
Hippocampus
Central Nervous System
Learning
Neurons
Water
Liver

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Selectively enhanced contextual fear conditioning in mice lacking the transcriptional regulator CCAAT/enhancer binding protein δ. / Sterneck, Esta; Paylor, Richard; Jackson-Lewis, Vernice; Libbey, Megan; Przedborski, Serge; Tessarollo, Lino; Crawley, Jacqueline; Johnson, Peter F.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, No. 18, 01.09.1998, p. 10908-10913.

Research output: Contribution to journalArticle

Sterneck, Esta ; Paylor, Richard ; Jackson-Lewis, Vernice ; Libbey, Megan ; Przedborski, Serge ; Tessarollo, Lino ; Crawley, Jacqueline ; Johnson, Peter F. / Selectively enhanced contextual fear conditioning in mice lacking the transcriptional regulator CCAAT/enhancer binding protein δ. In: Proceedings of the National Academy of Sciences of the United States of America. 1998 ; Vol. 95, No. 18. pp. 10908-10913.
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