Selective survival of peripheral blood lymphocytes in children with HIV-1 following delivery of an anti-HIV gene to bone marrow CD34+ cells

Greg M. Podsakoff, Barbara C. Engel, Denise A. Carbonaro, Chris Choi, Elzbieta M. Smogorzewska, Gerhard Bauer, David Selander, Susan Csik, Kathy Wilson, Michael R. Betts, Richard A. Koup, Gary J. Nabel, Keith Bishop, Steven King, Manfred Schmidt, Christof von Kalle, Joseph A. Church, Donald B. Kohn

Research output: Contribution to journalArticle

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Abstract

Two HIV-1-infected children on antiretroviral therapy were enrolled into a clinical study of retroviral-mediated transfer of a gene that inhibits replication of HIV-1, targeting bone marrow CD34+ hematopoietic stem/progenitor cells. Two retroviral vectors were used, one encoding a "humanized" dominant-negative REV protein (huM10) that is a potent inhibitor of HIV-1 replication and one encoding a nontranslated marker gene (FX) to serve as an internal control for the level of gene marking. Peripheral blood mononuclear cells (PBMC) containing the huM10 gene or FX gene were detected by quantitative PCR at frequencies of approximately 1/10,000 in both subjects for the first 1-3 months following re-infusion of the gene-transduced bone marrow, but then were at or below the limits of detection (<1/1,000,000) at most times over 2 years. In one patient, a reappearance of PBMC containing the huM10 gene, but not the FX gene, occurred concomitant with a rise in the HIV-1 viral load during a period of nonadherence to the 0antiretroviral regimen. Unique clones of gene-marked PBMC were detected by LAM-PCR during the time of elevated HIV-1 levels. These findings indicate that there was a selective survival advantage for PBMC containing the huM10 gene during the time of increased HIV-1 load.

Original languageEnglish (US)
Pages (from-to)77-86
Number of pages10
JournalMolecular Therapy
Volume12
Issue number1
DOIs
StatePublished - Jul 2005
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology

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    Podsakoff, G. M., Engel, B. C., Carbonaro, D. A., Choi, C., Smogorzewska, E. M., Bauer, G., Selander, D., Csik, S., Wilson, K., Betts, M. R., Koup, R. A., Nabel, G. J., Bishop, K., King, S., Schmidt, M., von Kalle, C., Church, J. A., & Kohn, D. B. (2005). Selective survival of peripheral blood lymphocytes in children with HIV-1 following delivery of an anti-HIV gene to bone marrow CD34+ cells. Molecular Therapy, 12(1), 77-86. https://doi.org/10.1016/j.ymthe.2005.02.024