Selective RNA editing and subunit assembly of native glutamate receptors

Ralph B. Puchalski, Jean Claude Louis, Nils Brose, Stephen F. Traynelis, Jan Egebjerg, Valery Kukekov, Robert J.Wenthold, Scott W. Rogers, Fan Lin, Thomas Moran, John Morrison, Stephen F. Heinemann

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

RNA editing and subunit assembly of ionotropic glutamate receptors (GIuRs) were examined in an oligodendrocyte progenitor cell line, CG4, which expresses GluR2-GluR4, GluR6, GluR7, KA1, and KA2. AMPA-evoked currents rapidly desensitize, whereas kainate-evoked currents contain a steady-state component with a nearly linear current-voltage relation and a fast desensitizing component that is inwardly rectifying. The Q/R site is edited >95% to the arginine codon in GluR2(Q607) mRNA, and <5% in GluR6(Q621) mRNA. Immunoprecipitation experiments demonstrate that GluR6 and/or GluR7 subunits assemble with KA2, but not with GluR2-GluR4. These results indicate that oligodendrocyte progenitor cells selectively edit and assemble glutamate receptors into at least two functionally and structurally distinct heteromeric channels.

Original languageEnglish (US)
Pages (from-to)131-147
Number of pages17
JournalNeuron
Volume13
Issue number1
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

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RNA Editing
Oligodendroglia
Glutamate Receptors
Stem Cells
Ionotropic Glutamate Receptors
Messenger RNA
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Kainic Acid
Immunoprecipitation
Codon
Arginine
Cell Line

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Puchalski, R. B., Louis, J. C., Brose, N., Traynelis, S. F., Egebjerg, J., Kukekov, V., ... Heinemann, S. F. (1994). Selective RNA editing and subunit assembly of native glutamate receptors. Neuron, 13(1), 131-147. https://doi.org/10.1016/0896-6273(94)90464-2

Selective RNA editing and subunit assembly of native glutamate receptors. / Puchalski, Ralph B.; Louis, Jean Claude; Brose, Nils; Traynelis, Stephen F.; Egebjerg, Jan; Kukekov, Valery; J.Wenthold, Robert; Rogers, Scott W.; Lin, Fan; Moran, Thomas; Morrison, John; Heinemann, Stephen F.

In: Neuron, Vol. 13, No. 1, 01.01.1994, p. 131-147.

Research output: Contribution to journalArticle

Puchalski, RB, Louis, JC, Brose, N, Traynelis, SF, Egebjerg, J, Kukekov, V, J.Wenthold, R, Rogers, SW, Lin, F, Moran, T, Morrison, J & Heinemann, SF 1994, 'Selective RNA editing and subunit assembly of native glutamate receptors', Neuron, vol. 13, no. 1, pp. 131-147. https://doi.org/10.1016/0896-6273(94)90464-2
Puchalski RB, Louis JC, Brose N, Traynelis SF, Egebjerg J, Kukekov V et al. Selective RNA editing and subunit assembly of native glutamate receptors. Neuron. 1994 Jan 1;13(1):131-147. https://doi.org/10.1016/0896-6273(94)90464-2
Puchalski, Ralph B. ; Louis, Jean Claude ; Brose, Nils ; Traynelis, Stephen F. ; Egebjerg, Jan ; Kukekov, Valery ; J.Wenthold, Robert ; Rogers, Scott W. ; Lin, Fan ; Moran, Thomas ; Morrison, John ; Heinemann, Stephen F. / Selective RNA editing and subunit assembly of native glutamate receptors. In: Neuron. 1994 ; Vol. 13, No. 1. pp. 131-147.
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