Selective photocleavage of DNA by anthraquinone derivatives: Targeting the single-strand region of hairpin structures

Paul Henderson, Bruce Armitage, Gary B. Schuster

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

A tetracationic anthraquinone derivative (27AQS2) binds to hairpin DNA and irradiation of the bound quinone leads to selective strand cleavage. NMR spectroscopy reveals that 27AQS2 binds at the loop and to the stem-loop junction of hairpin DNA. UV irradiation of the bound quinone causes cleavage of the DNA in the loop region and at guanines in the stem region. Inclusion of ethidium bromide in the reaction mixture leads to a greatly increased selectivity for loop cleavage. Spectroscopic and chemical evidence suggests a three component mechanism for reaction. The ability to target single-stranded regions of DNA structures is an important property of this photonuclease.

Original languageEnglish (US)
Pages (from-to)2991-3000
Number of pages10
JournalBiochemistry
Volume37
Issue number9
DOIs
StatePublished - Mar 3 1998
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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