A tetracationic anthraquinone derivative (27AQS2) binds to hairpin DNA and RNA. Ultraviolet irradiation of the bound quinone causes cleavage in the loop region of both oligonucleotides and at guanines in the stem region of the DNA hairpin. The absence of observable strand cleavage at guanines in the RNA hairpin suggests that either aniline treatment does not cause cleavage at damaged guanines in RNA or that radical cation migration does not occur readily in RNA duplexes. The ability to target the single-stranded regions of DNA and RNA structures is an important property of this photonuclease.
|Original language||English (US)|
|Number of pages||6|
|Journal||Photochemistry and Photobiology|
|State||Published - Dec 1999|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)