Selective interaction of voltage-gated K+ channel β-subunits with α-subunits

Kensuke Nakahira, Gongyi Shi, Kenneth J. Rhodes, James Trimmer

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Abstract

To begin to study the molecular bases that determine the selective interaction of the β-subunits of voltage-gated K+ channels with α-subunits observed in situ, we have expressed these polypeptides in transfected mammalian cells. Analysis of the specificity of α/β-subunit interaction indicates that both the Kvβ1 and Kvβ2 β-subunits display robust and selective interaction with the five members of the Shaker-related (Kv1) α-subunit subfamily tested. The interaction of these β-subunits with Kv1 α-subunits does not require the β-subunit N-terminal domains. Thus, the previously observed failure of N-terminal mutants of Kvβ1 to modulate inactivation kinetics of Kv1 family members is not simply due to a lack of subunit interaction. Interaction of these β-subunits with members of two other subfamilies (Shab- and Shaw-related) could not be detected. Somewhat surprisingly, a member of the Shal-related subfamily was found to interact with β-subunits; however, this interaction had biochemical characteristics distinct from the β-subunit interaction with Kv1 family members. In all cases, Kvβ1 and Kvβ2 exhibited indistinguishable α-subunit selectivity. These studies point to a selective interaction between K+ channel α- and β-subunits mediated through conserved domains in the respective subunits.

Original languageEnglish (US)
Pages (from-to)7084-7089
Number of pages6
JournalJournal of Biological Chemistry
Volume271
Issue number12
StatePublished - Mar 22 1996
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry

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Nakahira, K., Shi, G., Rhodes, K. J., & Trimmer, J. (1996). Selective interaction of voltage-gated K+ channel β-subunits with α-subunits. Journal of Biological Chemistry, 271(12), 7084-7089.