Selective inhibition of JH esterases from cockroach hemolymph

Bruce D. Hammock, Thomas C. Sparks, Susanne M. Mumby

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Juvenile hormone III was tritium labeled on the methyl ester and utilized with other substrates in an investigation of inhibition and substrate specificity of hemolymph esterases from the cockroach, Blaberus giganteus. The structure of labeled juvenile hormone III was supported both chemically and biochemically. Forty-two potential inhibitors were examined, and the best inhibitors included phosphoramidothiolates and S-phenylphosphates. One of these inhibitors was found useful in hormone biosynthesis studies dealing with the enzymatic conversion of methyl farnesoate to juvenile hormone in corpora allata homogenates. Several commonly used inhibitors of carboxyesterases caused only weak inhibition of JH esterases. Gel filtration elution patterns, inhibitor relationships, and specific activities of the hemolymph esterases indicate that juvenile hormones I and III are degraded by similar if not identical enzymes. In some cases, α-naphthyl acetate and juvenile hormone esterase activity could be differentially inhibited. Hemolymph esterases were not capable of degrading ethyl or isopropyl conjugated esters of two juvenoids or three model substrates.

Original languageEnglish (US)
Pages (from-to)517-530
Number of pages14
JournalPesticide Biochemistry and Physiology
Volume7
Issue number6
DOIs
StatePublished - 1977

Fingerprint

juvenile hormone esterase
Cockroaches
Hemolymph
juvenile hormones
Esterases
Blattodea
hemolymph
esterases
Esters
Substrates
Corpora Allata
Juvenile Hormones
Tritium
Biosynthesis
Blaberus giganteus
Substrate Specificity
esters
Gel Chromatography
Gels
juvenile hormone analogs

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Biochemistry
  • Physiology

Cite this

Selective inhibition of JH esterases from cockroach hemolymph. / Hammock, Bruce D.; Sparks, Thomas C.; Mumby, Susanne M.

In: Pesticide Biochemistry and Physiology, Vol. 7, No. 6, 1977, p. 517-530.

Research output: Contribution to journalArticle

Hammock, Bruce D. ; Sparks, Thomas C. ; Mumby, Susanne M. / Selective inhibition of JH esterases from cockroach hemolymph. In: Pesticide Biochemistry and Physiology. 1977 ; Vol. 7, No. 6. pp. 517-530.
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