Selective immobilization of proteins onto solid supports through split-intein-mediated protein trans-splicing

Youngeun Kwon, Matthew A Coleman, Julio A. Camarero

Research output: Contribution to journalArticle

88 Scopus citations

Abstract

(Figure Presented) Without a trace: The immobilization of biologically active proteins onto a solid support through split-intein-mediated protein trans-splicing reactions requires only very dilute samples. Not only is this process traceless but it does not require purification of the protein, thereby making possible the direct immobilization of proteins from complex mixtures such as cell lysates.

Original languageEnglish (US)
Pages (from-to)1726-1729
Number of pages4
JournalAngewandte Chemie - International Edition
Volume45
Issue number11
DOIs
StatePublished - Mar 3 2006
Externally publishedYes

Keywords

  • Bioorganic chemistry
  • Biosensors
  • Immobilization
  • Protein engineering
  • Proteomics

ASJC Scopus subject areas

  • Chemistry(all)

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