Selective expression of 15-lipoxygenase activity by cultured human keratinocytes

Barbara A Burrall, Bruce U. Wintroub, Edward J. Goetzl

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Human keratinocytes isolated from neonatal skin express 15-lipoxygenase activity at a level far greater than that of any of the other pathways for lipoxygenation of arachidonic acid. The 10,000 × g supernatant of sonicates of 106 keratinocytes generates 15-hydroxy-eicosatetraenoic acid from 5 μg/ml of arachidonic acid at a mean maximum rate of 38 ng/30 min at 37°C, that is similar to the activity of the 15-lipoxygenase of human airway epithelial cells and greater than that of endothelial cells and leukocytes. The unique mediators derived from the 15-lipoxygenation of arachidonic acid, that stimulate secretion and exert hyperalgesic effects, may achieve a concentration in skin sufficient to regulate local cellular and neural functions.

Original languageEnglish (US)
Pages (from-to)208-213
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume133
Issue number1
DOIs
StatePublished - Nov 27 1985

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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