Abstract
Human keratinocytes isolated from neonatal skin express 15-lipoxygenase activity at a level far greater than that of any of the other pathways for lipoxygenation of arachidonic acid. The 10,000 × g supernatant of sonicates of 106 keratinocytes generates 15-hydroxy-eicosatetraenoic acid from 5 μg/ml of arachidonic acid at a mean maximum rate of 38 ng/30 min at 37°C, that is similar to the activity of the 15-lipoxygenase of human airway epithelial cells and greater than that of endothelial cells and leukocytes. The unique mediators derived from the 15-lipoxygenation of arachidonic acid, that stimulate secretion and exert hyperalgesic effects, may achieve a concentration in skin sufficient to regulate local cellular and neural functions.
Original language | English (US) |
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Pages (from-to) | 208-213 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 133 |
Issue number | 1 |
DOIs | |
State | Published - Nov 27 1985 |
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology