Abstract
Human keratinocytes isolated from neonatal skin express 15-lipoxygenase activity at a level far greater than that of any of the other pathways for lipoxygenation of arachidonic acid. The 10,000 × g supernatant of sonicates of 106 keratinocytes generates 15-hydroxy-eicosatetraenoic acid from 5 μg/ml of arachidonic acid at a mean maximum rate of 38 ng/30 min at 37°C, that is similar to the activity of the 15-lipoxygenase of human airway epithelial cells and greater than that of endothelial cells and leukocytes. The unique mediators derived from the 15-lipoxygenation of arachidonic acid, that stimulate secretion and exert hyperalgesic effects, may achieve a concentration in skin sufficient to regulate local cellular and neural functions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 208-213 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 133 |
| Issue number | 1 |
| DOIs | |
| State | Published - Nov 27 1985 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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Selective expression of 15-lipoxygenase activity by cultured human keratinocytes. / Burrall, Barbara A; Wintroub, Bruce U.; Goetzl, Edward J.
In: Biochemical and Biophysical Research Communications, Vol. 133, No. 1, 27.11.1985, p. 208-213.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Selective expression of 15-lipoxygenase activity by cultured human keratinocytes
AU - Burrall, Barbara A
AU - Wintroub, Bruce U.
AU - Goetzl, Edward J.
PY - 1985/11/27
Y1 - 1985/11/27
N2 - Human keratinocytes isolated from neonatal skin express 15-lipoxygenase activity at a level far greater than that of any of the other pathways for lipoxygenation of arachidonic acid. The 10,000 × g supernatant of sonicates of 106 keratinocytes generates 15-hydroxy-eicosatetraenoic acid from 5 μg/ml of arachidonic acid at a mean maximum rate of 38 ng/30 min at 37°C, that is similar to the activity of the 15-lipoxygenase of human airway epithelial cells and greater than that of endothelial cells and leukocytes. The unique mediators derived from the 15-lipoxygenation of arachidonic acid, that stimulate secretion and exert hyperalgesic effects, may achieve a concentration in skin sufficient to regulate local cellular and neural functions.
AB - Human keratinocytes isolated from neonatal skin express 15-lipoxygenase activity at a level far greater than that of any of the other pathways for lipoxygenation of arachidonic acid. The 10,000 × g supernatant of sonicates of 106 keratinocytes generates 15-hydroxy-eicosatetraenoic acid from 5 μg/ml of arachidonic acid at a mean maximum rate of 38 ng/30 min at 37°C, that is similar to the activity of the 15-lipoxygenase of human airway epithelial cells and greater than that of endothelial cells and leukocytes. The unique mediators derived from the 15-lipoxygenation of arachidonic acid, that stimulate secretion and exert hyperalgesic effects, may achieve a concentration in skin sufficient to regulate local cellular and neural functions.
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UR - http://www.scopus.com/inward/citedby.url?scp=0022396027&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(85)91862-5
DO - 10.1016/0006-291X(85)91862-5
M3 - Article
C2 - 3935111
AN - SCOPUS:0022396027
VL - 133
SP - 208
EP - 213
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -