Selective expression of 15-lipoxygenase activity by cultured human keratinocytes

Barbara A Burrall, Bruce U. Wintroub, Edward J. Goetzl

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Human keratinocytes isolated from neonatal skin express 15-lipoxygenase activity at a level far greater than that of any of the other pathways for lipoxygenation of arachidonic acid. The 10,000 × g supernatant of sonicates of 106 keratinocytes generates 15-hydroxy-eicosatetraenoic acid from 5 μg/ml of arachidonic acid at a mean maximum rate of 38 ng/30 min at 37°C, that is similar to the activity of the 15-lipoxygenase of human airway epithelial cells and greater than that of endothelial cells and leukocytes. The unique mediators derived from the 15-lipoxygenation of arachidonic acid, that stimulate secretion and exert hyperalgesic effects, may achieve a concentration in skin sufficient to regulate local cellular and neural functions.

Original languageEnglish (US)
Pages (from-to)208-213
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume133
Issue number1
DOIs
StatePublished - Nov 27 1985

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Arachidonate 15-Lipoxygenase
Keratinocytes
Arachidonic Acid
Human Activities
Skin
Arachidonic Acids
Hydroxy Acids
Endothelial cells
Leukocytes
Endothelial Cells
Epithelial Cells

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Selective expression of 15-lipoxygenase activity by cultured human keratinocytes. / Burrall, Barbara A; Wintroub, Bruce U.; Goetzl, Edward J.

In: Biochemical and Biophysical Research Communications, Vol. 133, No. 1, 27.11.1985, p. 208-213.

Research output: Contribution to journalArticle

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