Selective expression of 15-lipoxygenase activity by cultured human keratinocytes

Barbara A Burrall; Bruce U. Wintroub; Edward J. Goetzl

doi:10.1016/0006-291X(85)91862-5

Selective expression of 15-lipoxygenase activity by cultured human keratinocytes

Barbara A Burrall, Bruce U. Wintroub, Edward J. Goetzl

Dermatology

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Human keratinocytes isolated from neonatal skin express 15-lipoxygenase activity at a level far greater than that of any of the other pathways for lipoxygenation of arachidonic acid. The 10,000 × g supernatant of sonicates of 10⁶ keratinocytes generates 15-hydroxy-eicosatetraenoic acid from 5 μg/ml of arachidonic acid at a mean maximum rate of 38 ng/30 min at 37°C, that is similar to the activity of the 15-lipoxygenase of human airway epithelial cells and greater than that of endothelial cells and leukocytes. The unique mediators derived from the 15-lipoxygenation of arachidonic acid, that stimulate secretion and exert hyperalgesic effects, may achieve a concentration in skin sufficient to regulate local cellular and neural functions.

Original language	English (US)
Pages (from-to)	208-213
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	133
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(85)91862-5
State	Published - Nov 27 1985

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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AU - Wintroub, Bruce U.

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AB - Human keratinocytes isolated from neonatal skin express 15-lipoxygenase activity at a level far greater than that of any of the other pathways for lipoxygenation of arachidonic acid. The 10,000 × g supernatant of sonicates of 106 keratinocytes generates 15-hydroxy-eicosatetraenoic acid from 5 μg/ml of arachidonic acid at a mean maximum rate of 38 ng/30 min at 37°C, that is similar to the activity of the 15-lipoxygenase of human airway epithelial cells and greater than that of endothelial cells and leukocytes. The unique mediators derived from the 15-lipoxygenation of arachidonic acid, that stimulate secretion and exert hyperalgesic effects, may achieve a concentration in skin sufficient to regulate local cellular and neural functions.

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Selective expression of 15-lipoxygenase activity by cultured human keratinocytes

Abstract

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