Human keratinocytes isolated from neonatal skin express 15-lipoxygenase activity at a level far greater than that of any of the other pathways for lipoxygenation of arachidonic acid. The 10,000 × g supernatant of sonicates of 106 keratinocytes generates 15-hydroxy-eicosatetraenoic acid from 5 μg/ml of arachidonic acid at a mean maximum rate of 38 ng/30 min at 37°C, that is similar to the activity of the 15-lipoxygenase of human airway epithelial cells and greater than that of endothelial cells and leukocytes. The unique mediators derived from the 15-lipoxygenation of arachidonic acid, that stimulate secretion and exert hyperalgesic effects, may achieve a concentration in skin sufficient to regulate local cellular and neural functions.
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Nov 27 1985|
ASJC Scopus subject areas
- Molecular Biology