Abstract
Intravenous injection of antigen-coupled splenocytes has been widely used to induce specific tolerance to a variety of antigens. In this study, we investigated the effects of such a treatment on Th1 and Th2 antigen-specific immune responses. Using both well-characterized model antigens and crude homogenates from Leishmania major promastigotes, we found that intravenous injection of antigen-coupled splenocytes strongly down-regulated antigen-specific Th2 responses but had no or only moderate effects on Th1 responses. Because the susceptibility of inbred strains of mice to murine leishmaniasis has been found to be correlated with a strong Th2 response against parasite antigens, we investigated whether administration of splenocytes chemically coupled to parasite antigens could protect susceptible mice from murine leishmaniasis. We found that this was indeed the case and further demonstrated that protection was associated with a strong decrease in the number of parasite-specific Th2-like cells. Because administration of antigen-coupled splenocytes is believed to induce ligation of the T cell receptor complex without inducing a co-stimulatory signal, our results further suggest that priming of Th1 cells is less dependent on co-stimulatory signals than the priming of Th2 cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 848-854 |
| Number of pages | 7 |
| Journal | European Journal of Immunology |
| Volume | 27 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1997 |
| Externally published | Yes |
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Keywords
- Immunotherapy
- In vivo animal model
- Infectious immunity-parasite
- Th1/Th2
- Tolerance/suppression
ASJC Scopus subject areas
- Immunology
Cite this
Selective down-regulation of Th2 immune responses following treatment with antigen-coupled splenocytes. / Soldera, Sylvelie; Mcsorley, Stephen J; Glaichenhaus, Nicolas.
In: European Journal of Immunology, Vol. 27, No. 4, 1997, p. 848-854.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Selective down-regulation of Th2 immune responses following treatment with antigen-coupled splenocytes
AU - Soldera, Sylvelie
AU - Mcsorley, Stephen J
AU - Glaichenhaus, Nicolas
PY - 1997
Y1 - 1997
N2 - Intravenous injection of antigen-coupled splenocytes has been widely used to induce specific tolerance to a variety of antigens. In this study, we investigated the effects of such a treatment on Th1 and Th2 antigen-specific immune responses. Using both well-characterized model antigens and crude homogenates from Leishmania major promastigotes, we found that intravenous injection of antigen-coupled splenocytes strongly down-regulated antigen-specific Th2 responses but had no or only moderate effects on Th1 responses. Because the susceptibility of inbred strains of mice to murine leishmaniasis has been found to be correlated with a strong Th2 response against parasite antigens, we investigated whether administration of splenocytes chemically coupled to parasite antigens could protect susceptible mice from murine leishmaniasis. We found that this was indeed the case and further demonstrated that protection was associated with a strong decrease in the number of parasite-specific Th2-like cells. Because administration of antigen-coupled splenocytes is believed to induce ligation of the T cell receptor complex without inducing a co-stimulatory signal, our results further suggest that priming of Th1 cells is less dependent on co-stimulatory signals than the priming of Th2 cells.
AB - Intravenous injection of antigen-coupled splenocytes has been widely used to induce specific tolerance to a variety of antigens. In this study, we investigated the effects of such a treatment on Th1 and Th2 antigen-specific immune responses. Using both well-characterized model antigens and crude homogenates from Leishmania major promastigotes, we found that intravenous injection of antigen-coupled splenocytes strongly down-regulated antigen-specific Th2 responses but had no or only moderate effects on Th1 responses. Because the susceptibility of inbred strains of mice to murine leishmaniasis has been found to be correlated with a strong Th2 response against parasite antigens, we investigated whether administration of splenocytes chemically coupled to parasite antigens could protect susceptible mice from murine leishmaniasis. We found that this was indeed the case and further demonstrated that protection was associated with a strong decrease in the number of parasite-specific Th2-like cells. Because administration of antigen-coupled splenocytes is believed to induce ligation of the T cell receptor complex without inducing a co-stimulatory signal, our results further suggest that priming of Th1 cells is less dependent on co-stimulatory signals than the priming of Th2 cells.
KW - Immunotherapy
KW - In vivo animal model
KW - Infectious immunity-parasite
KW - Th1/Th2
KW - Tolerance/suppression
UR - http://www.scopus.com/inward/record.url?scp=0030904082&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030904082&partnerID=8YFLogxK
U2 - 10.1002/eji.1830270407
DO - 10.1002/eji.1830270407
M3 - Article
C2 - 9130634
AN - SCOPUS:0030904082
VL - 27
SP - 848
EP - 854
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 4
ER -