Selective down-regulation of Th2 immune responses following treatment with antigen-coupled splenocytes

Sylvelie Soldera, Stephen J Mcsorley, Nicolas Glaichenhaus

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Intravenous injection of antigen-coupled splenocytes has been widely used to induce specific tolerance to a variety of antigens. In this study, we investigated the effects of such a treatment on Th1 and Th2 antigen-specific immune responses. Using both well-characterized model antigens and crude homogenates from Leishmania major promastigotes, we found that intravenous injection of antigen-coupled splenocytes strongly down-regulated antigen-specific Th2 responses but had no or only moderate effects on Th1 responses. Because the susceptibility of inbred strains of mice to murine leishmaniasis has been found to be correlated with a strong Th2 response against parasite antigens, we investigated whether administration of splenocytes chemically coupled to parasite antigens could protect susceptible mice from murine leishmaniasis. We found that this was indeed the case and further demonstrated that protection was associated with a strong decrease in the number of parasite-specific Th2-like cells. Because administration of antigen-coupled splenocytes is believed to induce ligation of the T cell receptor complex without inducing a co-stimulatory signal, our results further suggest that priming of Th1 cells is less dependent on co-stimulatory signals than the priming of Th2 cells.

Original languageEnglish (US)
Pages (from-to)848-854
Number of pages7
JournalEuropean Journal of Immunology
Volume27
Issue number4
DOIs
StatePublished - 1997
Externally publishedYes

Fingerprint

Down-Regulation
Antigens
Th2 Cells
Parasites
Leishmaniasis
Therapeutics
Intravenous Injections
Leishmania major
Th1 Cells
Inbred Strains Mice
Histocompatibility Antigens Class II
T-Cell Antigen Receptor
Ligation

Keywords

  • Immunotherapy
  • In vivo animal model
  • Infectious immunity-parasite
  • Th1/Th2
  • Tolerance/suppression

ASJC Scopus subject areas

  • Immunology

Cite this

Selective down-regulation of Th2 immune responses following treatment with antigen-coupled splenocytes. / Soldera, Sylvelie; Mcsorley, Stephen J; Glaichenhaus, Nicolas.

In: European Journal of Immunology, Vol. 27, No. 4, 1997, p. 848-854.

Research output: Contribution to journalArticle

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