Selective amplification of mouse mammary tumor virus in mammary tumors of GR mice

T. G. Fanning, J. P. Puma, Robert Cardiff

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

DNAs extracted from the mammary tumors of GR mice were analyzed for mouse mammary tumor virus proviral sequences by the restriction enzyme-Southern blot procedure. The tumor DNAs contain more proviral copies of mouse mammary tumor virus than DNA from a nonmalignant tissue. The degree of proviral amplification is small (ca. one to five additional copies) and appears to be variable from tumor to tumor. The restriction patterns of the amplified proviral sequences suggest a clonal origin for the tumor mass. In addition, the restriction patterns observed after digestion with the enzymes BglII and SacI indicate that only one of the proviruses endogenous to GR mice is amplified. The amplified provirus found in GR mammary tumors is identical to the provirus that is missing in GR-Mtv-2- mice, a congenic line exhibiting a low mammary tumor incidence.

Original languageEnglish (US)
Pages (from-to)109-114
Number of pages6
JournalJournal of Virology
Volume36
Issue number1
StatePublished - Jan 1 1980

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Mouse mammary tumor virus
mammary neoplasms (animal)
proviruses
Proviruses
Breast Neoplasms
neoplasms
mice
DNA
Neoplasms
Congenic Mice
Enzymes
enzymes
Southern Blotting
Southern blotting
Digestion
digestion
incidence
Incidence

ASJC Scopus subject areas

  • Immunology

Cite this

Selective amplification of mouse mammary tumor virus in mammary tumors of GR mice. / Fanning, T. G.; Puma, J. P.; Cardiff, Robert.

In: Journal of Virology, Vol. 36, No. 1, 01.01.1980, p. 109-114.

Research output: Contribution to journalArticle

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