Selection of anionic dopant for quantifying desialylation reactions with MALDI-FTMS

A. W. Wong, H. Wang, Carlito B Lebrilla

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Alkylsulfonates are examined as anion dopants for the matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) of neutral oligosaccharides. The anion dopants allow neutral oligosaccharides to be examined in the same mixture as acidic oligosaccharides. The alkylsulfonate dopants interact strongly with the oligosaccharide to produce a quasimolecular ion composed of the oligosaccharides and the deprotonated alkylsulfonates. Compounds as small as disaccharides to large branched oligosaccharides can all be examined. The new series of alkylsulfonates works as well as HSO4 - in terms of signal-to-noise ratio, sensitivity, and ease of preparation in the negative ion mode. The alkylsulfonates are used to construct a calibration curve for obtaining the relative amounts of varying neutral and acidic oligosaccharide mixtures. With the anion dopants, the MALDI-FTMS signals are shown to have a linear relationship with the molar ratios of the neutral and sialylated oligosaccharides. The method is also used to monitor the product of a desialylation reaction by acid hydrolysis.

Original languageEnglish (US)
Pages (from-to)1419-1425
Number of pages7
JournalAnalytical Chemistry
Volume72
Issue number7
DOIs
StatePublished - 2000

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Oligosaccharides
Doping (additives)
Anions
Disaccharides
Ionization
Mass spectrometry
Hydrolysis
Desorption
Signal to noise ratio
Negative ions
Calibration
Ions
Acids
Lasers

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Selection of anionic dopant for quantifying desialylation reactions with MALDI-FTMS. / Wong, A. W.; Wang, H.; Lebrilla, Carlito B.

In: Analytical Chemistry, Vol. 72, No. 7, 2000, p. 1419-1425.

Research output: Contribution to journalArticle

Wong, A. W. ; Wang, H. ; Lebrilla, Carlito B. / Selection of anionic dopant for quantifying desialylation reactions with MALDI-FTMS. In: Analytical Chemistry. 2000 ; Vol. 72, No. 7. pp. 1419-1425.
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N2 - Alkylsulfonates are examined as anion dopants for the matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) of neutral oligosaccharides. The anion dopants allow neutral oligosaccharides to be examined in the same mixture as acidic oligosaccharides. The alkylsulfonate dopants interact strongly with the oligosaccharide to produce a quasimolecular ion composed of the oligosaccharides and the deprotonated alkylsulfonates. Compounds as small as disaccharides to large branched oligosaccharides can all be examined. The new series of alkylsulfonates works as well as HSO4 - in terms of signal-to-noise ratio, sensitivity, and ease of preparation in the negative ion mode. The alkylsulfonates are used to construct a calibration curve for obtaining the relative amounts of varying neutral and acidic oligosaccharide mixtures. With the anion dopants, the MALDI-FTMS signals are shown to have a linear relationship with the molar ratios of the neutral and sialylated oligosaccharides. The method is also used to monitor the product of a desialylation reaction by acid hydrolysis.

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