Selection of an adenine resistant strain of Drosophila melanogaster with an altered form of xanthine dehydrogenase

Kevork Hagopian, E. J. Duke

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2 Scopus citations


A strain resistant to adenine (30 mM) has been selected from the wild-type Pac strain of Drosophila melanogaster. The resistant strain shows a prolonged life-cycle of 14 days, when cultured on adenine food, but reverts back to 9 days when cultured on normal food. The fecundity and fertility of the strain, when cultured on adenine food, are reduced by as much as 60%, but recover to the normal levels when the strain is cultured on normal food. Analysis of the stage distribution of mortalities show that the effective lethal phase (ELP) is larval. Analysis of the enzyme xanthine dehydrogenase from the adenine resistant strain revealed increases in the enzyme's activity, thermal stability and stability during dialysis, but no change in its molecular weight or electrophoretic mobility. The enzyme is also more stable with age and during prolonged incubation at 4°C. These alterations in the enzyme's properties were observed whether the flies were cultured on normal or adenine-containing food.

Original languageEnglish (US)
Pages (from-to)463-469
Number of pages7
JournalInsect Biochemistry
Issue number5
StatePublished - Jan 1 1989
Externally publishedYes



  • adenine
  • adenine resistance
  • Drosophila melanogaster
  • xanthine dehydrogenase

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