Abstract
We have investigated the usefulness of six in vitro biocompatibility tests in predicting the healing performance of polyester vascular prostheses as observed in previous canine in vivo trials. Vascular grafts were evaluated by using (i) a direct contact (DC) assay, (ii) an extract dilution (ED) assay on murine fibroblast cells, (iii) a DC assay on endothelial cells, (iv) a complement activation study, (v) a leucocyte activation study of CD18 integrin subunit expression on human polymorphonuclear cells (PMNs) and (vi) interleukin-2 receptor expression on lymphocytes. Uncleaned polyester grafts had previously been associated with poor healing and gelatin-impregnated polyester grafts with delayed but satisfactory healing, whereas commercially cleaned polyester grafts had demonstrated excellent healing. Lightweight and heavyweight knitted and woven polyester grafts supplied specifically for this project were studied, each with a different surface condition, i.e. commercially available (CP), uncleaned (UP) and impregnated with gelatin (GP). The UP grafts induced fibroblast cytotoxicity according to the ED assay, poor migration and viability of endothelial cells, and an elevated expression of CD18 and interleukin-2 receptor on PMNs and lymphocytes, respectively. In contrast, the CP grafts promoted good endothelial cell growth, no evidence of cytotoxicity and a weaker cell activation, and the GP grafts were found to be non-cytotoxic, to exhibit a good cellular response and to moderate cell activation. The complement activation assay and the DC assay on fibroblasts were found to be less useful and less discriminating. From this, it is concluded that the two cell activation measurements, the DC assay on endothelial cells and ED assay on fibroblasts, are useful in predicting the in vivo healing response of arterial polyester substitutes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1835-1842 |
| Number of pages | 8 |
| Journal | Biomaterials |
| Volume | 17 |
| Issue number | 19 |
| DOIs | |
| State | Published - Oct 1996 |
| Externally published | Yes |
Fingerprint
Keywords
- Biocompatibility
- Cell culture
- Endothelial cells
- Vascular prostheses
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biomedical Engineering
Cite this
Selecting valid in vitro biocompatibility tests that predict the in vivo healing response of synthetic vascular prostheses. / Marois, Yves; Guidoin, Robert; Roy, Raynald; Vidovszky, Tamas J; Jakubiec, Barbara; Sigot-Luizard, Marie Françoise; Braybrook, Julian; Mehri, Yahye; Laroche, Gaétan; King, Martin.
In: Biomaterials, Vol. 17, No. 19, 10.1996, p. 1835-1842.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Selecting valid in vitro biocompatibility tests that predict the in vivo healing response of synthetic vascular prostheses
AU - Marois, Yves
AU - Guidoin, Robert
AU - Roy, Raynald
AU - Vidovszky, Tamas J
AU - Jakubiec, Barbara
AU - Sigot-Luizard, Marie Françoise
AU - Braybrook, Julian
AU - Mehri, Yahye
AU - Laroche, Gaétan
AU - King, Martin
PY - 1996/10
Y1 - 1996/10
N2 - We have investigated the usefulness of six in vitro biocompatibility tests in predicting the healing performance of polyester vascular prostheses as observed in previous canine in vivo trials. Vascular grafts were evaluated by using (i) a direct contact (DC) assay, (ii) an extract dilution (ED) assay on murine fibroblast cells, (iii) a DC assay on endothelial cells, (iv) a complement activation study, (v) a leucocyte activation study of CD18 integrin subunit expression on human polymorphonuclear cells (PMNs) and (vi) interleukin-2 receptor expression on lymphocytes. Uncleaned polyester grafts had previously been associated with poor healing and gelatin-impregnated polyester grafts with delayed but satisfactory healing, whereas commercially cleaned polyester grafts had demonstrated excellent healing. Lightweight and heavyweight knitted and woven polyester grafts supplied specifically for this project were studied, each with a different surface condition, i.e. commercially available (CP), uncleaned (UP) and impregnated with gelatin (GP). The UP grafts induced fibroblast cytotoxicity according to the ED assay, poor migration and viability of endothelial cells, and an elevated expression of CD18 and interleukin-2 receptor on PMNs and lymphocytes, respectively. In contrast, the CP grafts promoted good endothelial cell growth, no evidence of cytotoxicity and a weaker cell activation, and the GP grafts were found to be non-cytotoxic, to exhibit a good cellular response and to moderate cell activation. The complement activation assay and the DC assay on fibroblasts were found to be less useful and less discriminating. From this, it is concluded that the two cell activation measurements, the DC assay on endothelial cells and ED assay on fibroblasts, are useful in predicting the in vivo healing response of arterial polyester substitutes.
AB - We have investigated the usefulness of six in vitro biocompatibility tests in predicting the healing performance of polyester vascular prostheses as observed in previous canine in vivo trials. Vascular grafts were evaluated by using (i) a direct contact (DC) assay, (ii) an extract dilution (ED) assay on murine fibroblast cells, (iii) a DC assay on endothelial cells, (iv) a complement activation study, (v) a leucocyte activation study of CD18 integrin subunit expression on human polymorphonuclear cells (PMNs) and (vi) interleukin-2 receptor expression on lymphocytes. Uncleaned polyester grafts had previously been associated with poor healing and gelatin-impregnated polyester grafts with delayed but satisfactory healing, whereas commercially cleaned polyester grafts had demonstrated excellent healing. Lightweight and heavyweight knitted and woven polyester grafts supplied specifically for this project were studied, each with a different surface condition, i.e. commercially available (CP), uncleaned (UP) and impregnated with gelatin (GP). The UP grafts induced fibroblast cytotoxicity according to the ED assay, poor migration and viability of endothelial cells, and an elevated expression of CD18 and interleukin-2 receptor on PMNs and lymphocytes, respectively. In contrast, the CP grafts promoted good endothelial cell growth, no evidence of cytotoxicity and a weaker cell activation, and the GP grafts were found to be non-cytotoxic, to exhibit a good cellular response and to moderate cell activation. The complement activation assay and the DC assay on fibroblasts were found to be less useful and less discriminating. From this, it is concluded that the two cell activation measurements, the DC assay on endothelial cells and ED assay on fibroblasts, are useful in predicting the in vivo healing response of arterial polyester substitutes.
KW - Biocompatibility
KW - Cell culture
KW - Endothelial cells
KW - Vascular prostheses
UR - http://www.scopus.com/inward/record.url?scp=0030269980&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030269980&partnerID=8YFLogxK
U2 - 10.1016/0142-9612(95)00245-6
DO - 10.1016/0142-9612(95)00245-6
M3 - Article
C2 - 8889062
AN - SCOPUS:0030269980
VL - 17
SP - 1835
EP - 1842
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 19
ER -