Selecting valid in vitro biocompatibility tests that predict the in vivo healing response of synthetic vascular prostheses

Yves Marois, Robert Guidoin, Raynald Roy, Tamas J Vidovszky, Barbara Jakubiec, Marie Françoise Sigot-Luizard, Julian Braybrook, Yahye Mehri, Gaétan Laroche, Martin King

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We have investigated the usefulness of six in vitro biocompatibility tests in predicting the healing performance of polyester vascular prostheses as observed in previous canine in vivo trials. Vascular grafts were evaluated by using (i) a direct contact (DC) assay, (ii) an extract dilution (ED) assay on murine fibroblast cells, (iii) a DC assay on endothelial cells, (iv) a complement activation study, (v) a leucocyte activation study of CD18 integrin subunit expression on human polymorphonuclear cells (PMNs) and (vi) interleukin-2 receptor expression on lymphocytes. Uncleaned polyester grafts had previously been associated with poor healing and gelatin-impregnated polyester grafts with delayed but satisfactory healing, whereas commercially cleaned polyester grafts had demonstrated excellent healing. Lightweight and heavyweight knitted and woven polyester grafts supplied specifically for this project were studied, each with a different surface condition, i.e. commercially available (CP), uncleaned (UP) and impregnated with gelatin (GP). The UP grafts induced fibroblast cytotoxicity according to the ED assay, poor migration and viability of endothelial cells, and an elevated expression of CD18 and interleukin-2 receptor on PMNs and lymphocytes, respectively. In contrast, the CP grafts promoted good endothelial cell growth, no evidence of cytotoxicity and a weaker cell activation, and the GP grafts were found to be non-cytotoxic, to exhibit a good cellular response and to moderate cell activation. The complement activation assay and the DC assay on fibroblasts were found to be less useful and less discriminating. From this, it is concluded that the two cell activation measurements, the DC assay on endothelial cells and ED assay on fibroblasts, are useful in predicting the in vivo healing response of arterial polyester substitutes.

Original languageEnglish (US)
Pages (from-to)1835-1842
Number of pages8
JournalBiomaterials
Volume17
Issue number19
DOIs
StatePublished - Oct 1996
Externally publishedYes

Fingerprint

Blood Vessel Prosthesis
Prosthetics
Biocompatibility
Grafts
Polyesters
Assays
Transplants
Chemical activation
Endothelial cells
Fibroblasts
Endothelial Cells
Dilution
Lymphocytes
Interleukin-2 Receptors
Complement Activation
Gelatin
Cytotoxicity
In Vitro Techniques
Cell growth
Cell Extracts

Keywords

  • Biocompatibility
  • Cell culture
  • Endothelial cells
  • Vascular prostheses

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Selecting valid in vitro biocompatibility tests that predict the in vivo healing response of synthetic vascular prostheses. / Marois, Yves; Guidoin, Robert; Roy, Raynald; Vidovszky, Tamas J; Jakubiec, Barbara; Sigot-Luizard, Marie Françoise; Braybrook, Julian; Mehri, Yahye; Laroche, Gaétan; King, Martin.

In: Biomaterials, Vol. 17, No. 19, 10.1996, p. 1835-1842.

Research output: Contribution to journalArticle

Marois, Y, Guidoin, R, Roy, R, Vidovszky, TJ, Jakubiec, B, Sigot-Luizard, MF, Braybrook, J, Mehri, Y, Laroche, G & King, M 1996, 'Selecting valid in vitro biocompatibility tests that predict the in vivo healing response of synthetic vascular prostheses', Biomaterials, vol. 17, no. 19, pp. 1835-1842. https://doi.org/10.1016/0142-9612(95)00245-6
Marois, Yves ; Guidoin, Robert ; Roy, Raynald ; Vidovszky, Tamas J ; Jakubiec, Barbara ; Sigot-Luizard, Marie Françoise ; Braybrook, Julian ; Mehri, Yahye ; Laroche, Gaétan ; King, Martin. / Selecting valid in vitro biocompatibility tests that predict the in vivo healing response of synthetic vascular prostheses. In: Biomaterials. 1996 ; Vol. 17, No. 19. pp. 1835-1842.
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AU - Jakubiec, Barbara

AU - Sigot-Luizard, Marie Françoise

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