Abstract
The random synthetic peptide library screening method based on the "one-bead one-peptide concept" (Selectide Process) can be used as a general method for the identification of peptide substrate motif for post-translational modifications. We applied this method to the cAMP-dependent protein kinase system and were able to rapidly identify a peptide motif (RRXS) that exactly matched that described for its natural substrates.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 401-403 |
| Number of pages | 3 |
| Journal | Methods |
| Volume | 6 |
| Issue number | 4 |
| DOIs | |
| State | Published - Dec 1994 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
Cite this
Selectide Technology : Substrate Motif Identification for Post-translational Modifications. / Lam, Kit; Wu, Jinzi.
In: Methods, Vol. 6, No. 4, 12.1994, p. 401-403.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Selectide Technology
T2 - Substrate Motif Identification for Post-translational Modifications
AU - Lam, Kit
AU - Wu, Jinzi
PY - 1994/12
Y1 - 1994/12
N2 - The random synthetic peptide library screening method based on the "one-bead one-peptide concept" (Selectide Process) can be used as a general method for the identification of peptide substrate motif for post-translational modifications. We applied this method to the cAMP-dependent protein kinase system and were able to rapidly identify a peptide motif (RRXS) that exactly matched that described for its natural substrates.
AB - The random synthetic peptide library screening method based on the "one-bead one-peptide concept" (Selectide Process) can be used as a general method for the identification of peptide substrate motif for post-translational modifications. We applied this method to the cAMP-dependent protein kinase system and were able to rapidly identify a peptide motif (RRXS) that exactly matched that described for its natural substrates.
UR - http://www.scopus.com/inward/record.url?scp=8244232090&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=8244232090&partnerID=8YFLogxK
U2 - 10.1006/meth.1994.1041
DO - 10.1006/meth.1994.1041
M3 - Article
AN - SCOPUS:8244232090
VL - 6
SP - 401
EP - 403
JO - Methods
JF - Methods
SN - 0076-6879
IS - 4
ER -