Secretion of insulin-like growth factor-II into bile of rats of different ages

Anthony F Philipps, Bohuslav Dvořák, Gary G. Anderson, James G. Grille, Catherine S. Williams, Otakar Koldovský

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Bile from rats of different ages (suckling 10-12 days; weanling 30-33 days, and adult 60-70 days) was collected and studied for the presence of immuno- and receptor-assayable insulin-like growth factor-II (IGF-II) concentrations. Concentrations of RIA IGF-II in bile were highest in suckling rats (230 ± 38 ng/ml) and lowest in adults (47 ± 7 ng/ml). These concentrations were approximately twice those of the bile IGF-I concentration in sucklings, as measured in a previous study. Selected bile samples were also assayed using a competitive binding assay with a crude preparation of adult rat liver membranes bearing the IGF-II receptor. These studies confirmed the presence of receptor- (as well as immuno-) active IGF-II in bile. Since bile flow rates increased dramatically after the suckling period, bile delivery rates of IGF-II were normalized as picograms per gram body weight per hour. When such calculations were done, bile IGF-II delivery rates to the small intestine were highest in sucklings and weanlings in comparison to adult rats. Thus non-enterically derived (milk- and bile-borne) IGF-II delivery to the suckling small intestine can be approximated at roughly 1 μg/day. Unlike IGF-I, intravenously injected IGF-II could not be detected in suckling bile, suggesting a predominantly hepatic origin. From this study we conclude that there exists a significant delivery of receptor-active IGF-II to the gastrointestinal tract of rats of all ages. Copyright (C) 2000 S. Karger AG, Basel.

Original languageEnglish (US)
Pages (from-to)106-112
Number of pages7
JournalBiology of the Neonate
Volume78
Issue number2
StatePublished - Aug 2000

Fingerprint

Insulin-Like Growth Factor II
Bile
IGF Type 2 Receptor
Insulin-Like Growth Factor I
Small Intestine
Competitive Binding
Liver
Gastrointestinal Tract
Milk
Body Weight

Keywords

  • Bile
  • Gastrointestinal tract
  • Insulin-like growth factors
  • Suckling

ASJC Scopus subject areas

  • Developmental Biology
  • Pediatrics, Perinatology, and Child Health

Cite this

Philipps, A. F., Dvořák, B., Anderson, G. G., Grille, J. G., Williams, C. S., & Koldovský, O. (2000). Secretion of insulin-like growth factor-II into bile of rats of different ages. Biology of the Neonate, 78(2), 106-112.

Secretion of insulin-like growth factor-II into bile of rats of different ages. / Philipps, Anthony F; Dvořák, Bohuslav; Anderson, Gary G.; Grille, James G.; Williams, Catherine S.; Koldovský, Otakar.

In: Biology of the Neonate, Vol. 78, No. 2, 08.2000, p. 106-112.

Research output: Contribution to journalArticle

Philipps, AF, Dvořák, B, Anderson, GG, Grille, JG, Williams, CS & Koldovský, O 2000, 'Secretion of insulin-like growth factor-II into bile of rats of different ages', Biology of the Neonate, vol. 78, no. 2, pp. 106-112.
Philipps AF, Dvořák B, Anderson GG, Grille JG, Williams CS, Koldovský O. Secretion of insulin-like growth factor-II into bile of rats of different ages. Biology of the Neonate. 2000 Aug;78(2):106-112.
Philipps, Anthony F ; Dvořák, Bohuslav ; Anderson, Gary G. ; Grille, James G. ; Williams, Catherine S. ; Koldovský, Otakar. / Secretion of insulin-like growth factor-II into bile of rats of different ages. In: Biology of the Neonate. 2000 ; Vol. 78, No. 2. pp. 106-112.
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