Secondhand tobacco smoke exposure differentially alters nucleus tractus solitarius neurons at two different ages in developing non-human primates

Shin ichi Sekizawa, Jesse P. Joad, Kent E Pinkerton, Ann C. Bonham

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Exposing children to secondhand tobacco smoke (SHS) is associated with increased risk for asthma, bronchiolitis and SIDS. The role for changes in the developing CNS contributing to these problems has not been fully explored. We used rhesus macaques to test the hypothesis that SHS exposure during development triggers neuroplastic changes in the nucleus tractus solitarius (NTS), where lung sensory information related to changes in airway and lung function is first integrated. Pregnant monkeys were exposed to filtered air (FA) or SHS for 6 h/day, 5 days/week starting at 50-day gestational age. Mother/infant pairs continued the exposures postnatally to age 3 or 13 months, which may be equivalent to approximately 1 or 4 years of human age, respectively. Whole-cell recordings were made of second-order NTS neurons in transverse brainstem slices. To target the consequences of SHS exposure based on neuronal subgroups, we classified NTS neurons into two phenotypes, rapid-onset spiking (RS) and delayed-onset spiking (DS), and then evaluated intrinsic and synaptic excitabilities in FA-exposed animals. RS neurons showed greater cell excitability especially at age of 3 months while DS neurons received greater amplitudes of excitatory postsynaptic currents (EPSCs). Developmental neuroplasticity such as increases in intrinsic and synaptic excitabilities were detected especially in DS neurons. In 3 month olds, SHS exposure effects were limited to excitatory changes in RS neurons, specifically increases in evoked EPSC amplitudes and increased spiking responses accompanied by shortened action potential width. By 13 months, the continued SHS exposure inhibited DS neuronal activity; decreases in evoked EPSC amplitudes and blunted spiking responses accompanied by prolonged action potential width. The influence of SHS exposure on age-related and phenotype specific changes may be associated with age-specific respiratory problems, for which SHS exposure can increase the risk, such as SIDS and bronchiolitis in infants and asthma in older children.

Original languageEnglish (US)
Pages (from-to)199-208
Number of pages10
JournalToxicology and Applied Pharmacology
Volume242
Issue number2
DOIs
StatePublished - Jan 15 2010

Fingerprint

Tobacco Smoke Pollution
Solitary Nucleus
Tobacco
Smoke
Primates
Neurons
Excitatory Postsynaptic Potentials
Bronchiolitis
Sudden Infant Death
Action Potentials
Asthma
Air
Phenotype
Lung
Neuronal Plasticity
Patch-Clamp Techniques
Macaca mulatta
Gestational Age
Brain Stem
Haplorhini

Keywords

  • Airway sensory afferents
  • Indoor air pollutant
  • Infant
  • Intrinsic cell excitability
  • Nucleus tractus solitarius
  • Primates
  • Respiration
  • Secondhand smoke
  • Synaptic transmission

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Secondhand tobacco smoke exposure differentially alters nucleus tractus solitarius neurons at two different ages in developing non-human primates. / Sekizawa, Shin ichi; Joad, Jesse P.; Pinkerton, Kent E; Bonham, Ann C.

In: Toxicology and Applied Pharmacology, Vol. 242, No. 2, 15.01.2010, p. 199-208.

Research output: Contribution to journalArticle

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