TY - JOUR
T1 - SecB dependence of an exported protein is a continuum influenced by the characteristics of the signal peptide or early mature region
AU - Kim, Jinoh
AU - Luirink, Joen
AU - Kendall, Debra A.
PY - 2000/7
Y1 - 2000/7
N2 - We have used Escherichia coli alkaline phosphatase to show the interplay among the characteristics of two amino-terminal domains in the preprotein (the signal peptide and the early mature region), the efficiency with which this protein is transported, and its requirement for SecB to accomplish the transport process. The results suggest that although alkaline phosphatase does not normally require SecB for transport, it is inherently able to utilize SecB, and it does so when its ability to interface with the transport machinery is compromised.
AB - We have used Escherichia coli alkaline phosphatase to show the interplay among the characteristics of two amino-terminal domains in the preprotein (the signal peptide and the early mature region), the efficiency with which this protein is transported, and its requirement for SecB to accomplish the transport process. The results suggest that although alkaline phosphatase does not normally require SecB for transport, it is inherently able to utilize SecB, and it does so when its ability to interface with the transport machinery is compromised.
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U2 - 10.1128/JB.182.14.4108-4112.2000
DO - 10.1128/JB.182.14.4108-4112.2000
M3 - Article
C2 - 10869093
AN - SCOPUS:0033945367
VL - 182
SP - 4108
EP - 4112
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 14
ER -