Screening small-molecule compound microarrays for protein ligands without fluorescence labeling with a high-throughput scanning microscope

Yiyan Fei; James P. Landry; Yungshin Sun; Xiangdong Zhu; Xiaobing Wang; Juntao Luo; Chun Yi Wu; Kit Lam

doi:10.1117/1.3309743

Screening small-molecule compound microarrays for protein ligands without fluorescence labeling with a high-throughput scanning microscope

Yiyan Fei, James P. Landry, Yungshin Sun, Xiangdong Zhu, Xiaobing Wang, Juntao Luo, Chun Yi Wu, Kit Lam

Research output: Contribution to journal › Article

26 Scopus citations

Abstract

We describe a high-throughput scanning optical microscope for detecting small-molecule compound microarrays on functionalized glass slides. It is based on measurements of obliqueincidence reflectivity difference and employs a combination of a y-scan galvometer mirror and an x-scan translation stage with an effective field of view of 2 cm × 4 cm. Such a field of view can accommodate a printed small-molecule compound microarray with as many as 10,000 to 20,000 targets. The scanning microscope is capable of measuring kinetics as well as endpoints of protein-ligand reactions simultaneously. We present the experimental results on solutionphase protein reactions with small-molecule compound microarrays synthesized from one-bead, one-compound combinatorial chemistry and immobilized on a streptavidin-functionalized glass slide.

Original language	English (US)
Article number	016018
Journal	Journal of Biomedical Optics
Volume	15
Issue number	1
DOIs	https://doi.org/10.1117/1.3309743
State	Published - 2010

Keywords

High-throughput
Immobilization strategies
In situ detection
Label-free detection
Microarrays
Oblique-incidence reflectivity difference

ASJC Scopus subject areas

Biomedical Engineering
Biomaterials
Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics

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Fei, Y., Landry, J. P., Sun, Y., Zhu, X., Wang, X., Luo, J., Wu, C. Y., & Lam, K. (2010). Screening small-molecule compound microarrays for protein ligands without fluorescence labeling with a high-throughput scanning microscope. Journal of Biomedical Optics, 15(1), [016018]. https://doi.org/10.1117/1.3309743

Screening small-molecule compound microarrays for protein ligands without fluorescence labeling with a high-throughput scanning microscope. / Fei, Yiyan; Landry, James P.; Sun, Yungshin; Zhu, Xiangdong; Wang, Xiaobing; Luo, Juntao; Wu, Chun Yi; Lam, Kit.

In: Journal of Biomedical Optics, Vol. 15, No. 1, 016018, 2010.

Research output: Contribution to journal › Article

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