Abstract
We describe a high-throughput scanning optical microscope for detecting small-molecule compound microarrays on functionalized glass slides. It is based on measurements of obliqueincidence reflectivity difference and employs a combination of a y-scan galvometer mirror and an x-scan translation stage with an effective field of view of 2 cm × 4 cm. Such a field of view can accommodate a printed small-molecule compound microarray with as many as 10,000 to 20,000 targets. The scanning microscope is capable of measuring kinetics as well as endpoints of protein-ligand reactions simultaneously. We present the experimental results on solutionphase protein reactions with small-molecule compound microarrays synthesized from one-bead, one-compound combinatorial chemistry and immobilized on a streptavidin-functionalized glass slide.
| Original language | English (US) |
|---|---|
| Article number | 016018 |
| Journal | Journal of Biomedical Optics |
| Volume | 15 |
| Issue number | 1 |
| DOIs | |
| State | Published - 2010 |
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Keywords
- High-throughput
- Immobilization strategies
- In situ detection
- Label-free detection
- Microarrays
- Oblique-incidence reflectivity difference
ASJC Scopus subject areas
- Biomedical Engineering
- Biomaterials
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
Cite this
Screening small-molecule compound microarrays for protein ligands without fluorescence labeling with a high-throughput scanning microscope. / Fei, Yiyan; Landry, James P.; Sun, Yungshin; Zhu, Xiangdong; Wang, Xiaobing; Luo, Juntao; Wu, Chun Yi; Lam, Kit.
In: Journal of Biomedical Optics, Vol. 15, No. 1, 016018, 2010.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Screening small-molecule compound microarrays for protein ligands without fluorescence labeling with a high-throughput scanning microscope
AU - Fei, Yiyan
AU - Landry, James P.
AU - Sun, Yungshin
AU - Zhu, Xiangdong
AU - Wang, Xiaobing
AU - Luo, Juntao
AU - Wu, Chun Yi
AU - Lam, Kit
PY - 2010
Y1 - 2010
N2 - We describe a high-throughput scanning optical microscope for detecting small-molecule compound microarrays on functionalized glass slides. It is based on measurements of obliqueincidence reflectivity difference and employs a combination of a y-scan galvometer mirror and an x-scan translation stage with an effective field of view of 2 cm × 4 cm. Such a field of view can accommodate a printed small-molecule compound microarray with as many as 10,000 to 20,000 targets. The scanning microscope is capable of measuring kinetics as well as endpoints of protein-ligand reactions simultaneously. We present the experimental results on solutionphase protein reactions with small-molecule compound microarrays synthesized from one-bead, one-compound combinatorial chemistry and immobilized on a streptavidin-functionalized glass slide.
AB - We describe a high-throughput scanning optical microscope for detecting small-molecule compound microarrays on functionalized glass slides. It is based on measurements of obliqueincidence reflectivity difference and employs a combination of a y-scan galvometer mirror and an x-scan translation stage with an effective field of view of 2 cm × 4 cm. Such a field of view can accommodate a printed small-molecule compound microarray with as many as 10,000 to 20,000 targets. The scanning microscope is capable of measuring kinetics as well as endpoints of protein-ligand reactions simultaneously. We present the experimental results on solutionphase protein reactions with small-molecule compound microarrays synthesized from one-bead, one-compound combinatorial chemistry and immobilized on a streptavidin-functionalized glass slide.
KW - High-throughput
KW - Immobilization strategies
KW - In situ detection
KW - Label-free detection
KW - Microarrays
KW - Oblique-incidence reflectivity difference
UR - http://www.scopus.com/inward/record.url?scp=77953160917&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953160917&partnerID=8YFLogxK
U2 - 10.1117/1.3309743
DO - 10.1117/1.3309743
M3 - Article
C2 - 20210464
AN - SCOPUS:77953160917
VL - 15
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
SN - 1083-3668
IS - 1
M1 - 016018
ER -