Abstract
We describe a high-throughput scanning optical microscope for detecting small-molecule compound microarrays on functionalized glass slides. It is based on measurements of obliqueincidence reflectivity difference and employs a combination of a y-scan galvometer mirror and an x-scan translation stage with an effective field of view of 2 cm × 4 cm. Such a field of view can accommodate a printed small-molecule compound microarray with as many as 10,000 to 20,000 targets. The scanning microscope is capable of measuring kinetics as well as endpoints of protein-ligand reactions simultaneously. We present the experimental results on solutionphase protein reactions with small-molecule compound microarrays synthesized from one-bead, one-compound combinatorial chemistry and immobilized on a streptavidin-functionalized glass slide.
Original language | English (US) |
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Article number | 016018 |
Journal | Journal of Biomedical Optics |
Volume | 15 |
Issue number | 1 |
DOIs | |
State | Published - 2010 |
Keywords
- High-throughput
- Immobilization strategies
- In situ detection
- Label-free detection
- Microarrays
- Oblique-incidence reflectivity difference
ASJC Scopus subject areas
- Biomedical Engineering
- Biomaterials
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics