Screening small-molecule compound microarrays for protein ligands without fluorescence labeling with a high-throughput scanning microscope

Yiyan Fei, James P. Landry, Yungshin Sun, Xiangdong Zhu, Xiaobing Wang, Juntao Luo, Chun Yi Wu, Kit Lam

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

We describe a high-throughput scanning optical microscope for detecting small-molecule compound microarrays on functionalized glass slides. It is based on measurements of obliqueincidence reflectivity difference and employs a combination of a y-scan galvometer mirror and an x-scan translation stage with an effective field of view of 2 cm × 4 cm. Such a field of view can accommodate a printed small-molecule compound microarray with as many as 10,000 to 20,000 targets. The scanning microscope is capable of measuring kinetics as well as endpoints of protein-ligand reactions simultaneously. We present the experimental results on solutionphase protein reactions with small-molecule compound microarrays synthesized from one-bead, one-compound combinatorial chemistry and immobilized on a streptavidin-functionalized glass slide.

Original languageEnglish (US)
Article number016018
JournalJournal of Biomedical Optics
Volume15
Issue number1
DOIs
StatePublished - 2010

Keywords

  • High-throughput
  • Immobilization strategies
  • In situ detection
  • Label-free detection
  • Microarrays
  • Oblique-incidence reflectivity difference

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

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