Abstract
Canine lower urinary tract neoplasia is a clinically important disease process that has high mortality due to late stage diagnosis and poorly durable response to treatment. Non-invasive diagnostic techniques (e.g. dipstick test, urine cytology) currently have poor diagnostic value, while more invasive tests (e.g. cystoscopy and biopsy) are costly and often require general anesthesia. We have developed and herein describe a quantitative cytological analysis method based on the use of surface-enhanced Raman spectroscopy (SERS), for identifying cancerous transitional cells in urine using SERS biotags (SBTs) carrying the peptide PLZ4 (amino acid sequence cQDGRMGFc) that targets malignant transitional cells. By analyzing the ratio of the PLZ4-SBTs to an on board control we were able to show that transitional cells had significantly higher ratios (P < 0.05) in patients diagnosed with transitional cell carcinoma (TCC) than in healthy samples.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1279-1287 |
| Number of pages | 9 |
| Journal | Nanomedicine: Nanotechnology, Biology, and Medicine |
| Volume | 14 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jun 1 2018 |
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Keywords
- Cancer detection
- Liquid biopsy
- Surface enhanced Raman spectroscopy (SERS)
- Transitional cell carcinoma (TCC)
- Urine
ASJC Scopus subject areas
- Bioengineering
- Medicine (miscellaneous)
- Molecular Medicine
- Biomedical Engineering
- Materials Science(all)
- Pharmaceutical Science
Cite this
Screening for canine transitional cell carcinoma (TCC) by SERS-based quantitative urine cytology. / Pallaoro, Alessia; Mirsafavi, Rustin Y.; Culp, William T; Braun, Gary B.; Meinhart, Carl D.; Moskovits, Martin.
In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 14, No. 4, 01.06.2018, p. 1279-1287.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Screening for canine transitional cell carcinoma (TCC) by SERS-based quantitative urine cytology
AU - Pallaoro, Alessia
AU - Mirsafavi, Rustin Y.
AU - Culp, William T
AU - Braun, Gary B.
AU - Meinhart, Carl D.
AU - Moskovits, Martin
PY - 2018/6/1
Y1 - 2018/6/1
N2 - Canine lower urinary tract neoplasia is a clinically important disease process that has high mortality due to late stage diagnosis and poorly durable response to treatment. Non-invasive diagnostic techniques (e.g. dipstick test, urine cytology) currently have poor diagnostic value, while more invasive tests (e.g. cystoscopy and biopsy) are costly and often require general anesthesia. We have developed and herein describe a quantitative cytological analysis method based on the use of surface-enhanced Raman spectroscopy (SERS), for identifying cancerous transitional cells in urine using SERS biotags (SBTs) carrying the peptide PLZ4 (amino acid sequence cQDGRMGFc) that targets malignant transitional cells. By analyzing the ratio of the PLZ4-SBTs to an on board control we were able to show that transitional cells had significantly higher ratios (P < 0.05) in patients diagnosed with transitional cell carcinoma (TCC) than in healthy samples.
AB - Canine lower urinary tract neoplasia is a clinically important disease process that has high mortality due to late stage diagnosis and poorly durable response to treatment. Non-invasive diagnostic techniques (e.g. dipstick test, urine cytology) currently have poor diagnostic value, while more invasive tests (e.g. cystoscopy and biopsy) are costly and often require general anesthesia. We have developed and herein describe a quantitative cytological analysis method based on the use of surface-enhanced Raman spectroscopy (SERS), for identifying cancerous transitional cells in urine using SERS biotags (SBTs) carrying the peptide PLZ4 (amino acid sequence cQDGRMGFc) that targets malignant transitional cells. By analyzing the ratio of the PLZ4-SBTs to an on board control we were able to show that transitional cells had significantly higher ratios (P < 0.05) in patients diagnosed with transitional cell carcinoma (TCC) than in healthy samples.
KW - Cancer detection
KW - Liquid biopsy
KW - Surface enhanced Raman spectroscopy (SERS)
KW - Transitional cell carcinoma (TCC)
KW - Urine
UR - http://www.scopus.com/inward/record.url?scp=85046791238&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85046791238&partnerID=8YFLogxK
U2 - 10.1016/j.nano.2018.03.001
DO - 10.1016/j.nano.2018.03.001
M3 - Article
C2 - 29597048
AN - SCOPUS:85046791238
VL - 14
SP - 1279
EP - 1287
JO - Nanomedicine: Nanotechnology, Biology, and Medicine
JF - Nanomedicine: Nanotechnology, Biology, and Medicine
SN - 1549-9634
IS - 4
ER -