Screening and Confirmatory Analyses of Flunixin in Tissues and Bodily Fluids after Intravenous or Intramuscular Administration to Cull Dairy Cows with or without Lipopolysaccharide Challenge

Weilin L. Shelver, David J. Smith, Lisa A Tell, Ronald E. Baynes, J. W. Schroeder, Jim E. Riviere

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Twenty cull dairy cows (645 ± 83 kg) were treated with 2.2 mg/kg bw flunixin by intravenous (IV) or intramuscular (IM) administration with, or without, exposure to lipopolysaccharide in a two factor balanced design. The usefulness of screening assays to identify violative flunixin levels in a variety of easily accessible ante-mortem fluids in cattle was explored. Two animals with violative flunixin liver residue and/or violative 5-hydroxy flunixin milk residues were correctly identified by a flunixin liver ELISA screen. Oral fluid did not produce anticipated flunixin concentration profiles using ELISA determination. One cow that had liver and milk violative residues, and one cow that had a milk violation at the prescribed withdrawal period were correctly identified by flunixin milk lateral flow analyses. The ratio of urinary flunixin and 5-hydroxy flunixin may be useful for predicting disruption of metabolism caused by disease or other factors potentially leading to violative liver flunixin residues.

Original languageEnglish (US)
Pages (from-to)336-345
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume64
Issue number1
DOIs
StatePublished - Jan 13 2016

Keywords

  • concentration
  • ELISA
  • flunixin
  • food safety
  • route of administration

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

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