Scintigraphic Tracking of Allogeneic Mesenchymal Stem Cells in the Distal Limb After Intra-Arterial Injection in Standing Horses

Pablo Espinosa, Mathieu Spriet, Albert Sole, Naomi J. Walker, Mary E Vaughan, Larry D Galuppo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objective: To assess the feasibility of intra-arterial administration of allogeneic mesenchymal stem cells (MSC) in the median artery of standing horses and evaluate the distribution and retention of radiolabeled cells. Study Design: In vivo experimental study. Animals: Six research horses. Methods: Technetium99m-HexaMethyl-Propylene-Amine Oxime-labeled MSC were injected under ultrasound guidance in the median artery of 6 front limbs of 3 horses, standing under sedation. Scintigraphic images were obtained at the time of injection, and at 1, 6, and 24 hours postinjection. Six additional limbs from 3 horses were similarly injected with unlabeled MSC. Ultrasound was performed the following day for assessment of vascular changes. Results: Intra-arterial injection was performed successfully in 11 of 12 limbs. In 1 limb, partial periarterial injection compromised the success of the procedure. Homogeneous distribution of radiolabeled MSC was observed through the entire distal limb, including within the hoof. Partial venous thrombosis was found in both groups of horses, but was subjectively less severe in horses injected with unlabeled MSC. No lameness was observed. Transient swelling of the distal limb occurred in only 1 limb. Conclusion: Intra-arterial injection of MSC can be performed in standing horses under sedation and successfully distribute MSC to the distal limb. A risk of periarterial injection was identified but can be reduced with proper sedation, local anesthesia, and increased experience. Partial venous thrombosis was observed as a complication, but did not cause changes of clinical importance, other than rare transient swelling.

Original languageEnglish (US)
Pages (from-to)619-624
Number of pages6
JournalVeterinary Surgery
Volume45
Issue number5
DOIs
StatePublished - Jul 1 2016

Fingerprint

Intra-Arterial Injections
limbs (animal)
Mesenchymal Stromal Cells
Horses
stem cells
Extremities
injection
horses
sedation
thrombosis
Venous Thrombosis
arteries
Injections
Arteries
propylene
oximes
Hoof and Claw
Oximes
hooves
Local Anesthesia

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Scintigraphic Tracking of Allogeneic Mesenchymal Stem Cells in the Distal Limb After Intra-Arterial Injection in Standing Horses. / Espinosa, Pablo; Spriet, Mathieu; Sole, Albert; Walker, Naomi J.; Vaughan, Mary E; Galuppo, Larry D.

In: Veterinary Surgery, Vol. 45, No. 5, 01.07.2016, p. 619-624.

Research output: Contribution to journalArticle

@article{f040f7f6c80c4d45a9d9d1f82e0e15d9,
title = "Scintigraphic Tracking of Allogeneic Mesenchymal Stem Cells in the Distal Limb After Intra-Arterial Injection in Standing Horses",
abstract = "Objective: To assess the feasibility of intra-arterial administration of allogeneic mesenchymal stem cells (MSC) in the median artery of standing horses and evaluate the distribution and retention of radiolabeled cells. Study Design: In vivo experimental study. Animals: Six research horses. Methods: Technetium99m-HexaMethyl-Propylene-Amine Oxime-labeled MSC were injected under ultrasound guidance in the median artery of 6 front limbs of 3 horses, standing under sedation. Scintigraphic images were obtained at the time of injection, and at 1, 6, and 24 hours postinjection. Six additional limbs from 3 horses were similarly injected with unlabeled MSC. Ultrasound was performed the following day for assessment of vascular changes. Results: Intra-arterial injection was performed successfully in 11 of 12 limbs. In 1 limb, partial periarterial injection compromised the success of the procedure. Homogeneous distribution of radiolabeled MSC was observed through the entire distal limb, including within the hoof. Partial venous thrombosis was found in both groups of horses, but was subjectively less severe in horses injected with unlabeled MSC. No lameness was observed. Transient swelling of the distal limb occurred in only 1 limb. Conclusion: Intra-arterial injection of MSC can be performed in standing horses under sedation and successfully distribute MSC to the distal limb. A risk of periarterial injection was identified but can be reduced with proper sedation, local anesthesia, and increased experience. Partial venous thrombosis was observed as a complication, but did not cause changes of clinical importance, other than rare transient swelling.",
author = "Pablo Espinosa and Mathieu Spriet and Albert Sole and Walker, {Naomi J.} and Vaughan, {Mary E} and Galuppo, {Larry D}",
year = "2016",
month = "7",
day = "1",
doi = "10.1111/vsu.12485",
language = "English (US)",
volume = "45",
pages = "619--624",
journal = "Veterinary Surgery",
issn = "0161-3499",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Scintigraphic Tracking of Allogeneic Mesenchymal Stem Cells in the Distal Limb After Intra-Arterial Injection in Standing Horses

AU - Espinosa, Pablo

AU - Spriet, Mathieu

AU - Sole, Albert

AU - Walker, Naomi J.

AU - Vaughan, Mary E

AU - Galuppo, Larry D

PY - 2016/7/1

Y1 - 2016/7/1

N2 - Objective: To assess the feasibility of intra-arterial administration of allogeneic mesenchymal stem cells (MSC) in the median artery of standing horses and evaluate the distribution and retention of radiolabeled cells. Study Design: In vivo experimental study. Animals: Six research horses. Methods: Technetium99m-HexaMethyl-Propylene-Amine Oxime-labeled MSC were injected under ultrasound guidance in the median artery of 6 front limbs of 3 horses, standing under sedation. Scintigraphic images were obtained at the time of injection, and at 1, 6, and 24 hours postinjection. Six additional limbs from 3 horses were similarly injected with unlabeled MSC. Ultrasound was performed the following day for assessment of vascular changes. Results: Intra-arterial injection was performed successfully in 11 of 12 limbs. In 1 limb, partial periarterial injection compromised the success of the procedure. Homogeneous distribution of radiolabeled MSC was observed through the entire distal limb, including within the hoof. Partial venous thrombosis was found in both groups of horses, but was subjectively less severe in horses injected with unlabeled MSC. No lameness was observed. Transient swelling of the distal limb occurred in only 1 limb. Conclusion: Intra-arterial injection of MSC can be performed in standing horses under sedation and successfully distribute MSC to the distal limb. A risk of periarterial injection was identified but can be reduced with proper sedation, local anesthesia, and increased experience. Partial venous thrombosis was observed as a complication, but did not cause changes of clinical importance, other than rare transient swelling.

AB - Objective: To assess the feasibility of intra-arterial administration of allogeneic mesenchymal stem cells (MSC) in the median artery of standing horses and evaluate the distribution and retention of radiolabeled cells. Study Design: In vivo experimental study. Animals: Six research horses. Methods: Technetium99m-HexaMethyl-Propylene-Amine Oxime-labeled MSC were injected under ultrasound guidance in the median artery of 6 front limbs of 3 horses, standing under sedation. Scintigraphic images were obtained at the time of injection, and at 1, 6, and 24 hours postinjection. Six additional limbs from 3 horses were similarly injected with unlabeled MSC. Ultrasound was performed the following day for assessment of vascular changes. Results: Intra-arterial injection was performed successfully in 11 of 12 limbs. In 1 limb, partial periarterial injection compromised the success of the procedure. Homogeneous distribution of radiolabeled MSC was observed through the entire distal limb, including within the hoof. Partial venous thrombosis was found in both groups of horses, but was subjectively less severe in horses injected with unlabeled MSC. No lameness was observed. Transient swelling of the distal limb occurred in only 1 limb. Conclusion: Intra-arterial injection of MSC can be performed in standing horses under sedation and successfully distribute MSC to the distal limb. A risk of periarterial injection was identified but can be reduced with proper sedation, local anesthesia, and increased experience. Partial venous thrombosis was observed as a complication, but did not cause changes of clinical importance, other than rare transient swelling.

UR - http://www.scopus.com/inward/record.url?scp=84977493074&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84977493074&partnerID=8YFLogxK

U2 - 10.1111/vsu.12485

DO - 10.1111/vsu.12485

M3 - Article

C2 - 27246971

AN - SCOPUS:84977493074

VL - 45

SP - 619

EP - 624

JO - Veterinary Surgery

JF - Veterinary Surgery

SN - 0161-3499

IS - 5

ER -