Abstract
Surface galactocerebro side (galC) was induced on cultured Schwann cells by two analogues of cyclic adenosine 3′,5′-monophosphate (cAMP), dibutyryl cAMP and 8-bromo cAMP (as previously reported by Sobue and Pleasure31) and also by forskolin, a potent adenylate cyclase activator. These reagents also induced a morphological transition of many of the Schwann cells, from an elongated spindle shape of flattened cells extending fenestrated cytoplasmic sheets. Surface galC and these changes in Schwann cell shape were not elicited by rising the extracellular cAMP concentration, nor by many compounds known to promote the differentiation of other cell types, suggesting that intracellular cAMP is the unique signal for their induction. The cAMP analogues also induced Schwann cell proliferation (as previously reported by Raff et al.26), as did forskolin. The concentrations of cAMP analogues and forskolin eliciting largest increases in numbers of Schwann cells in the cultures were 10-fold lower than the concentrations required for optimal induction of Schwann cell surface galC.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 23-32 |
| Number of pages | 10 |
| Journal | Brain Research |
| Volume | 362 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1986 |
| Externally published | Yes |
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Keywords
- cyclic adenosine 3′5′-monophosphate
- galactocerebroside
- myelination
- neuroglial proliferation
- Schwann cell
ASJC Scopus subject areas
- Developmental Biology
- Molecular Biology
- Clinical Neurology
- Neuroscience(all)
Cite this
Schwann cell responses to cyclic AMP : Proliferation, change in shape, and appearance of surface galactocerebroside. / Sobue, Gen; Shuman, Sandra; Pleasure, David E.
In: Brain Research, Vol. 362, No. 1, 01.01.1986, p. 23-32.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Schwann cell responses to cyclic AMP
T2 - Proliferation, change in shape, and appearance of surface galactocerebroside
AU - Sobue, Gen
AU - Shuman, Sandra
AU - Pleasure, David E
PY - 1986/1/1
Y1 - 1986/1/1
N2 - Surface galactocerebro side (galC) was induced on cultured Schwann cells by two analogues of cyclic adenosine 3′,5′-monophosphate (cAMP), dibutyryl cAMP and 8-bromo cAMP (as previously reported by Sobue and Pleasure31) and also by forskolin, a potent adenylate cyclase activator. These reagents also induced a morphological transition of many of the Schwann cells, from an elongated spindle shape of flattened cells extending fenestrated cytoplasmic sheets. Surface galC and these changes in Schwann cell shape were not elicited by rising the extracellular cAMP concentration, nor by many compounds known to promote the differentiation of other cell types, suggesting that intracellular cAMP is the unique signal for their induction. The cAMP analogues also induced Schwann cell proliferation (as previously reported by Raff et al.26), as did forskolin. The concentrations of cAMP analogues and forskolin eliciting largest increases in numbers of Schwann cells in the cultures were 10-fold lower than the concentrations required for optimal induction of Schwann cell surface galC.
AB - Surface galactocerebro side (galC) was induced on cultured Schwann cells by two analogues of cyclic adenosine 3′,5′-monophosphate (cAMP), dibutyryl cAMP and 8-bromo cAMP (as previously reported by Sobue and Pleasure31) and also by forskolin, a potent adenylate cyclase activator. These reagents also induced a morphological transition of many of the Schwann cells, from an elongated spindle shape of flattened cells extending fenestrated cytoplasmic sheets. Surface galC and these changes in Schwann cell shape were not elicited by rising the extracellular cAMP concentration, nor by many compounds known to promote the differentiation of other cell types, suggesting that intracellular cAMP is the unique signal for their induction. The cAMP analogues also induced Schwann cell proliferation (as previously reported by Raff et al.26), as did forskolin. The concentrations of cAMP analogues and forskolin eliciting largest increases in numbers of Schwann cells in the cultures were 10-fold lower than the concentrations required for optimal induction of Schwann cell surface galC.
KW - cyclic adenosine 3′5′-monophosphate
KW - galactocerebroside
KW - myelination
KW - neuroglial proliferation
KW - Schwann cell
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UR - http://www.scopus.com/inward/citedby.url?scp=0022631190&partnerID=8YFLogxK
U2 - 10.1016/0006-8993(86)91394-6
DO - 10.1016/0006-8993(86)91394-6
M3 - Article
C2 - 3002553
AN - SCOPUS:0022631190
VL - 362
SP - 23
EP - 32
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1
ER -