Schwann cell responses to cyclic AMP: Proliferation, change in shape, and appearance of surface galactocerebroside

Gen Sobue, Sandra Shuman, David E Pleasure

Research output: Contribution to journalArticle

125 Scopus citations

Abstract

Surface galactocerebro side (galC) was induced on cultured Schwann cells by two analogues of cyclic adenosine 3′,5′-monophosphate (cAMP), dibutyryl cAMP and 8-bromo cAMP (as previously reported by Sobue and Pleasure31) and also by forskolin, a potent adenylate cyclase activator. These reagents also induced a morphological transition of many of the Schwann cells, from an elongated spindle shape of flattened cells extending fenestrated cytoplasmic sheets. Surface galC and these changes in Schwann cell shape were not elicited by rising the extracellular cAMP concentration, nor by many compounds known to promote the differentiation of other cell types, suggesting that intracellular cAMP is the unique signal for their induction. The cAMP analogues also induced Schwann cell proliferation (as previously reported by Raff et al.26), as did forskolin. The concentrations of cAMP analogues and forskolin eliciting largest increases in numbers of Schwann cells in the cultures were 10-fold lower than the concentrations required for optimal induction of Schwann cell surface galC.

Original languageEnglish (US)
Pages (from-to)23-32
Number of pages10
JournalBrain Research
Volume362
Issue number1
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

Keywords

  • cyclic adenosine 3′5′-monophosphate
  • galactocerebroside
  • myelination
  • neuroglial proliferation
  • Schwann cell

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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