Abstract
Indirect immunofluorescence was used to show the presence of galactocerebroside (galC), a lipid found in myelin, on the surface of about half of the Schwann cells isolated from neonatal rat sciatic nerves and cultured for 1 day without neurons. By day 4 in vitro, the Schwann cells had all lost their surface galC. Three days after beginning treatment with 10-3 molar 8-bromo-adenosine 3′,5′-monophosphate (8-bromo cyclic AMP) or N 6,O2-dibutyryl adenosine 3′,5′-monophosphate (dibutyryl cyclic AMP), galC reappeared on the Schwann cells, and 2 days later 48 percent of the cultured Schwann cells showed surface galC. Tritium from tritiated D-galactose was incorporated into galC by the 8-bromo cyclic AMP-and dibutyryl cyclic AMP-treated cultures at a rate 15 times the control rate.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 72-74 |
| Number of pages | 3 |
| Journal | Science |
| Volume | 224 |
| Issue number | 4644 |
| State | Published - 1984 |
| Externally published | Yes |
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Cite this
Schwann cell galactocerebroside induced by derivatives of adenosine 3′,5′-monophosphate. / Sobue, Gen; Pleasure, David E.
In: Science, Vol. 224, No. 4644, 1984, p. 72-74.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Schwann cell galactocerebroside induced by derivatives of adenosine 3′,5′-monophosphate
AU - Sobue, Gen
AU - Pleasure, David E
PY - 1984
Y1 - 1984
N2 - Indirect immunofluorescence was used to show the presence of galactocerebroside (galC), a lipid found in myelin, on the surface of about half of the Schwann cells isolated from neonatal rat sciatic nerves and cultured for 1 day without neurons. By day 4 in vitro, the Schwann cells had all lost their surface galC. Three days after beginning treatment with 10-3 molar 8-bromo-adenosine 3′,5′-monophosphate (8-bromo cyclic AMP) or N 6,O2-dibutyryl adenosine 3′,5′-monophosphate (dibutyryl cyclic AMP), galC reappeared on the Schwann cells, and 2 days later 48 percent of the cultured Schwann cells showed surface galC. Tritium from tritiated D-galactose was incorporated into galC by the 8-bromo cyclic AMP-and dibutyryl cyclic AMP-treated cultures at a rate 15 times the control rate.
AB - Indirect immunofluorescence was used to show the presence of galactocerebroside (galC), a lipid found in myelin, on the surface of about half of the Schwann cells isolated from neonatal rat sciatic nerves and cultured for 1 day without neurons. By day 4 in vitro, the Schwann cells had all lost their surface galC. Three days after beginning treatment with 10-3 molar 8-bromo-adenosine 3′,5′-monophosphate (8-bromo cyclic AMP) or N 6,O2-dibutyryl adenosine 3′,5′-monophosphate (dibutyryl cyclic AMP), galC reappeared on the Schwann cells, and 2 days later 48 percent of the cultured Schwann cells showed surface galC. Tritium from tritiated D-galactose was incorporated into galC by the 8-bromo cyclic AMP-and dibutyryl cyclic AMP-treated cultures at a rate 15 times the control rate.
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M3 - Article
VL - 224
SP - 72
EP - 74
JO - Science
JF - Science
SN - 0036-8075
IS - 4644
ER -