Schizosaccharomyces pombe fatty acid synthase mediates DNA strand exchange in vitro

Edgar Käslin, Wolf Dietrich Heyer

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

During purification of a strand exchange activity from Schizosaccharomyces pombe using the three-strand reaction of double-stranded linear and circular single-stranded DNA, we identified p190/210 as an activity that stimulated the strand exchange activity of p140(exo2) by about 10-fold. The accompanying report (Kaslin, E., and Heyer, W.-D. (1994) J. Biol. Chem. 269, 0000-0000) described the purification and characterization of p140(exo2), likely to be the S. pombe homolog of the Saccharomyces cerevisiae strand exchange protein p175(SEP)1. Here, we report the purification of p190/210 from S. pombe cells and its identification as fatty acid synthase (FAS). S. pombe FAS (p190/210) binds to single-stranded and double-stranded DNA, leading to condensation of DNA into large aggregates. In addition, it is capable of renaturing complementary single-stranded DNA. Besides stimulating the strand exchange activity of p140(exo2), FAS (p190/210) itself exhibits strand exchange activity provided the double-stranded substrate has single-stranded tails. We propose a probable mechanism for the action of FAS (p190/210) during DNA strand exchange in vitro. Since FAS (p190/210) is highly unlikely to have a role in homologous recombination in vivo, we discuss the implications of our data on the interpretation of other homologous pairing and strand exchange proteins purified from eukaryotes using this or similar assays.

Original languageEnglish (US)
Pages (from-to)14103-14110
Number of pages8
JournalJournal of Biological Chemistry
Volume269
Issue number19
StatePublished - May 13 1994
Externally publishedYes

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Fatty Acid Synthases
Schizosaccharomyces
Ion exchange
DNA
Purification
Single-Stranded DNA
Circular DNA
Homologous Recombination
Eukaryota
Yeast
Saccharomyces cerevisiae
Condensation
Assays
Proteins
Complementary DNA
In Vitro Techniques
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Schizosaccharomyces pombe fatty acid synthase mediates DNA strand exchange in vitro. / Käslin, Edgar; Heyer, Wolf Dietrich.

In: Journal of Biological Chemistry, Vol. 269, No. 19, 13.05.1994, p. 14103-14110.

Research output: Contribution to journalArticle

Käslin, Edgar ; Heyer, Wolf Dietrich. / Schizosaccharomyces pombe fatty acid synthase mediates DNA strand exchange in vitro. In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 19. pp. 14103-14110.
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