Scavenger cells with Gram-positive bacterial lipoteichoic acid infiltrate around the damaged interlobular bile ducts of primary biliary cirrhosis

Koichi Tsuneyama, Kenichi Harada, Naoko Kono, Katsushi Hiramatsu, Yoh Zen, Yoshiko Sudo, M. Eric Gershwin, Mamoru Ikemoto, Hiroyuki Arai, Yasuni Nakanuma

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

Background/Aims: Gram-positive bacterial DNA is frequently detectable in gallbladder bile of primary biliary cirrhosis (PBC) patients. To advance these findings, lipoteichoic acid (LTA) of Gram-positive bacteria with high antigenicity was examined in liver specimens and bile from PBC patients and controls. Methods: LTA was examined by Western blotting in the gallbladder bile from 15 PBC, 11 cholecystolithiasis and six normal subjects, and by immunohistochemistry in liver specimens from 16 PBC, six primary sclerosing cholangitis (PSC), eight chronic viral hepatitis C (CVH-C) and five normal subjects. Results: In the gallbladder bile, there was no significant difference in the positive rate of LTA between PBC and controls. LTA-containing mononuclear cells were frequently detected in the portal tracts, particularly around the bile ducts and in hepatic sinusoids in PBC, while they were infrequent or occasional in control livers. These LTA-containing cells were sinusoidal endothelial cells and Kupffer cells, and portal monocytes, which frequently expressed scavenger receptor class B type 1. Conclusions: LTA derived from bacterial fragments may reach the bile, not only in the diseased state but also under normal conditions. Such LTA may be involved in the development and progression of portal tract lesions, particularly bile duct lesions, in PBC.

Original languageEnglish (US)
Pages (from-to)156-163
Number of pages8
JournalJournal of Hepatology
Volume35
Issue number2
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Gram-positive bacteria
  • Granuloma
  • Interlobular bile duct
  • Lipoteichoic acid
  • Primary biliary cirrhosis

ASJC Scopus subject areas

  • Gastroenterology

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