Satratoxin-G from the black mold stachybotrys chartarum induces rhinitis and apoptosis of olfactory sensory neurons in the nasal airways of rhesus monkeys

Stephan A. Carey, Charles Plopper, Dallas M. Hyde, Zahidul Islam, James J. Pestka, Jack R. Harkema

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Satratoxin-G (SG) is a trichothecene mycotoxin of Stachybotrys chartarum, the black mold suggested to contribute etiologically to illnesses associated with water-damaged buildings. We have reported that intranasal exposure to SG evokes apoptosis of olfactory sensory neurons (OSNs) and acute inflammation in the nose and brain of laboratory mice. To further assess the potential human risk of nasal airway injury and neurotoxicity, we developed a model of SG exposure in monkeys, whose nasal airways more closely resemble those of humans. Adult, male rhesus macaques received a single intranasal instillation of 20 μg SG (high dose, n = 3), or 5 μg SG daily for four days (repeated low dose, n = 3) in one nasal passage, and saline vehicle in the contralateral nasal passage. Nasal tissues were examined using light and electron microscopy and morphometric analysis. SG induced acute rhinitis, atrophy of the olfactory epithelium (OE), and apoptosis of OSNs in both groups. High-dose and repeated low-dose SG elicited a 13% and 66% reduction in OSN volume density, and a 14-fold and 24-fold increase in apoptotic cells of the OE, respectively. This model provides new insight into the potential risk of nasal airway injury and neurotoxicity caused by exposure to water-damaged buildings.

Original languageEnglish (US)
Pages (from-to)887-898
Number of pages12
JournalToxicologic Pathology
Volume40
Issue number6
DOIs
StatePublished - Aug 2012

Fingerprint

Stachybotrys
Olfactory Receptor Neurons
Rhinitis
Macaca mulatta
Nose
Neurons
Fungi
Apoptosis
Olfactory Mucosa
Trichothecenes
Water
Mycotoxins
Wounds and Injuries
satratoxin G
Electron microscopy
Atrophy
Optical microscopy
Haplorhini
Brain
Electron Microscopy

Keywords

  • mycotoxin
  • olfactory toxicity
  • rhesus monkeys
  • stachybotrys
  • trichothecene

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology
  • Cell Biology
  • Molecular Biology

Cite this

Satratoxin-G from the black mold stachybotrys chartarum induces rhinitis and apoptosis of olfactory sensory neurons in the nasal airways of rhesus monkeys. / Carey, Stephan A.; Plopper, Charles; Hyde, Dallas M.; Islam, Zahidul; Pestka, James J.; Harkema, Jack R.

In: Toxicologic Pathology, Vol. 40, No. 6, 08.2012, p. 887-898.

Research output: Contribution to journalArticle

Carey, Stephan A. ; Plopper, Charles ; Hyde, Dallas M. ; Islam, Zahidul ; Pestka, James J. ; Harkema, Jack R. / Satratoxin-G from the black mold stachybotrys chartarum induces rhinitis and apoptosis of olfactory sensory neurons in the nasal airways of rhesus monkeys. In: Toxicologic Pathology. 2012 ; Vol. 40, No. 6. pp. 887-898.
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