Sampling of Intestinal Microbiota and Targeted Amplification of Bacterial 16S rRNA Genes for Microbial Ecologic Analysis

Maomeng Tong, Jonathan P. Jacobs, Ian Howard Mchardy, Jonathan Braun

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Dysbiosis of host-associated commensal microbiota is emerging as an important factor in risk and phenotype of immunologic, metabolic, and behavioral diseases. Accurate analysis of microbial composition and functional state in humans or mice requires appropriate collection and pre-processing of biospecimens. Methods to sample luminal and mucosal microbiota from human or mouse intestines and to profile microbial phylogenetic composition using 16S rRNA sequencing are presented here. Data generated using the methods in this unit can be used for downstream quantitative analysis of microbial ecology.

Original languageEnglish (US)
Pages (from-to)7.41.1-7.41.11
JournalCurrent Protocols in Immunology
Volume2014
DOIs
StatePublished - 2014

Keywords

  • 16S rRNA
  • Illumina sequencing
  • Intestinal microbiota
  • Lavage
  • Mucosal microbiota

ASJC Scopus subject areas

  • Immunology

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