Sam68 sequestration and partial loss of function are associated with splicing alterations in FXTAS patients

Chantal Sellier, Frédérique Rau, Yilei Liu, Flora Tassone, Renate K. Hukema, Renata Gattoni, Anne Schneider, Stéphane Richard, Rob Willemsen, David J. Elliott, Paul J Hagerman, Nicolas Charlet-Berguerand

Research output: Contribution to journalArticle

209 Citations (Scopus)

Abstract

Fragile X-associated Tremor/Ataxia Syndrome (FXTAS) is a neurodegenerative disorder caused by expansion of 55-200 CGG repeats in the 5′-UTR of the FMR1 gene. FXTAS is characterized by action tremor, gait ataxia and impaired executive cognitive functioning. It has been proposed that FXTAS is caused by titration of RNA-binding proteins by the expanded CGG repeats. Sam68 is an RNA-binding protein involved in alternative splicing regulation and its ablation in mouse leads to motor coordination defects. Here, we report that mRNAs containing expanded CGG repeats form large and dynamic intranuclear RNA aggregates that recruit several RNA-binding proteins sequentially, first Sam68, then hnRNP-G and MBNL1. Importantly, Sam68 is sequestered by expanded CGG repeats and thereby loses its splicing-regulatory function. Consequently, Sam68-responsive splicing is altered in FXTAS patients. Finally, we found that regulation of Sam68 tyrosine phosphorylation modulates its localization within CGG aggregates and that tautomycin prevents both Sam68 and CGG RNA aggregate formation. Overall, these data support an RNA gain-of-function mechanism for FXTAS neuropathology, and suggest possible target routes for treatment options.

Original languageEnglish (US)
Pages (from-to)1248-1261
Number of pages14
JournalEMBO Journal
Volume29
Issue number7
DOIs
StatePublished - Apr 2010

Fingerprint

RNA-Binding Proteins
RNA
Heterogeneous-Nuclear Ribonucleoproteins
Phosphorylation
5' Untranslated Regions
Alternative Splicing
Ablation
Titration
Gait Ataxia
Tyrosine
Genes
Tremor
Messenger RNA
Defects
Neurodegenerative Diseases
Fragile X Tremor Ataxia Syndrome

Keywords

  • FXTAS
  • RNA gain-of-function diseases
  • Sam68

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Sellier, C., Rau, F., Liu, Y., Tassone, F., Hukema, R. K., Gattoni, R., ... Charlet-Berguerand, N. (2010). Sam68 sequestration and partial loss of function are associated with splicing alterations in FXTAS patients. EMBO Journal, 29(7), 1248-1261. https://doi.org/10.1038/emboj.2010.21

Sam68 sequestration and partial loss of function are associated with splicing alterations in FXTAS patients. / Sellier, Chantal; Rau, Frédérique; Liu, Yilei; Tassone, Flora; Hukema, Renate K.; Gattoni, Renata; Schneider, Anne; Richard, Stéphane; Willemsen, Rob; Elliott, David J.; Hagerman, Paul J; Charlet-Berguerand, Nicolas.

In: EMBO Journal, Vol. 29, No. 7, 04.2010, p. 1248-1261.

Research output: Contribution to journalArticle

Sellier, C, Rau, F, Liu, Y, Tassone, F, Hukema, RK, Gattoni, R, Schneider, A, Richard, S, Willemsen, R, Elliott, DJ, Hagerman, PJ & Charlet-Berguerand, N 2010, 'Sam68 sequestration and partial loss of function are associated with splicing alterations in FXTAS patients', EMBO Journal, vol. 29, no. 7, pp. 1248-1261. https://doi.org/10.1038/emboj.2010.21
Sellier, Chantal ; Rau, Frédérique ; Liu, Yilei ; Tassone, Flora ; Hukema, Renate K. ; Gattoni, Renata ; Schneider, Anne ; Richard, Stéphane ; Willemsen, Rob ; Elliott, David J. ; Hagerman, Paul J ; Charlet-Berguerand, Nicolas. / Sam68 sequestration and partial loss of function are associated with splicing alterations in FXTAS patients. In: EMBO Journal. 2010 ; Vol. 29, No. 7. pp. 1248-1261.
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abstract = "Fragile X-associated Tremor/Ataxia Syndrome (FXTAS) is a neurodegenerative disorder caused by expansion of 55-200 CGG repeats in the 5′-UTR of the FMR1 gene. FXTAS is characterized by action tremor, gait ataxia and impaired executive cognitive functioning. It has been proposed that FXTAS is caused by titration of RNA-binding proteins by the expanded CGG repeats. Sam68 is an RNA-binding protein involved in alternative splicing regulation and its ablation in mouse leads to motor coordination defects. Here, we report that mRNAs containing expanded CGG repeats form large and dynamic intranuclear RNA aggregates that recruit several RNA-binding proteins sequentially, first Sam68, then hnRNP-G and MBNL1. Importantly, Sam68 is sequestered by expanded CGG repeats and thereby loses its splicing-regulatory function. Consequently, Sam68-responsive splicing is altered in FXTAS patients. Finally, we found that regulation of Sam68 tyrosine phosphorylation modulates its localization within CGG aggregates and that tautomycin prevents both Sam68 and CGG RNA aggregate formation. Overall, these data support an RNA gain-of-function mechanism for FXTAS neuropathology, and suggest possible target routes for treatment options.",
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