Abstract
NAD can serve as both a purine and a pyridine source for Salmonella typhimurium. Exogenous NAD is rapidly broken down into nicotinamide mononucleotide and AMP by an NAD pyrophosphatase, the first step in the pathway for the assimilation of exogenous NAD. We isolated and characterized mutants of S. typhimurium lacking NAD pyrophosphatase activity; such mutants were identified by their failure to use exogenous NAD as a purine source. These mutants carry mutations that map at a new locus, designated pnuE, between 86 and 87 min on the Salmonella chromosome.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3725-3730 |
| Number of pages | 6 |
| Journal | Journal of Bacteriology |
| Volume | 170 |
| Issue number | 8 |
| State | Published - 1988 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Immunology
Cite this
Salmonella typhimurium mutants lacking NAD pyrophosphatase. / Park, U. E.; Roth, J. R.; Olivera, B. M.
In: Journal of Bacteriology, Vol. 170, No. 8, 1988, p. 3725-3730.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Salmonella typhimurium mutants lacking NAD pyrophosphatase
AU - Park, U. E.
AU - Roth, J. R.
AU - Olivera, B. M.
PY - 1988
Y1 - 1988
N2 - NAD can serve as both a purine and a pyridine source for Salmonella typhimurium. Exogenous NAD is rapidly broken down into nicotinamide mononucleotide and AMP by an NAD pyrophosphatase, the first step in the pathway for the assimilation of exogenous NAD. We isolated and characterized mutants of S. typhimurium lacking NAD pyrophosphatase activity; such mutants were identified by their failure to use exogenous NAD as a purine source. These mutants carry mutations that map at a new locus, designated pnuE, between 86 and 87 min on the Salmonella chromosome.
AB - NAD can serve as both a purine and a pyridine source for Salmonella typhimurium. Exogenous NAD is rapidly broken down into nicotinamide mononucleotide and AMP by an NAD pyrophosphatase, the first step in the pathway for the assimilation of exogenous NAD. We isolated and characterized mutants of S. typhimurium lacking NAD pyrophosphatase activity; such mutants were identified by their failure to use exogenous NAD as a purine source. These mutants carry mutations that map at a new locus, designated pnuE, between 86 and 87 min on the Salmonella chromosome.
UR - http://www.scopus.com/inward/record.url?scp=0023782394&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023782394&partnerID=8YFLogxK
M3 - Article
C2 - 2841298
AN - SCOPUS:0023782394
VL - 170
SP - 3725
EP - 3730
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 8
ER -