Salmonella typhimurium loci involved in survival within macrophages

Andreas J Baumler, J. G. Kusters, I. Stojiljkovic, F. Heffron

Research output: Contribution to journalArticle

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Abstract

A set of Tn10 mutants of Salmonella typhimurium which have a diminished capacity to survive in murine macrophages and decreased virulence in mice has been described previously. In this study, we characterized 30 of these mutants and determined map locations of Tn10 insertions for 23 of these strains. In addition, short fragments of transposon-flanking DNA were cloned, and the nucleotide sequence was determined for 23 mutants. Seven mutants carried transposon insertions in known genes, representing six loci: htrA, prc, purD, fliD, nagA, and smpB. The possible roles of these genes in Salmonella virulence are discussed. One insertion was found to be in an unknown gene which shared homology with the open reading frames Bv' and Bv located in the pin inversion system of Shigella boydii. In one mutant, Tn10 was found to be inserted in a gene with significant homology to adhE of Escherichia coli and Clostridium acetobutylicum. The map location and degree of homology indicate that the Salmonella gene encodes a related, but different, dehydrogenase. In 14 of the mutants analyzed, Tn10 was inserted into genes which had no significant homologies to entries in the DNA and protein data bases. In conclusion, 16 insertions define loci, termed ims for impaired macrophage survival, which have not yet been described in S. typhimurium but have been shown previously to be necessary for full virulence in mice. Although most ims loci are distributed randomly throughout the genome, a cluster was found between 75 and 78 min on the Salmonella chromosome.

Original languageEnglish (US)
Pages (from-to)1623-1630
Number of pages8
JournalInfection and Immunity
Volume62
Issue number5
StatePublished - 1994
Externally publishedYes

Fingerprint

Salmonella typhimurium
Macrophages
Salmonella
Genes
Virulence
Shigella boydii
Clostridium acetobutylicum
DNA Transposable Elements
Open Reading Frames
Oxidoreductases
Chromosomes
Genome
Databases
Escherichia coli
DNA
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Baumler, A. J., Kusters, J. G., Stojiljkovic, I., & Heffron, F. (1994). Salmonella typhimurium loci involved in survival within macrophages. Infection and Immunity, 62(5), 1623-1630.

Salmonella typhimurium loci involved in survival within macrophages. / Baumler, Andreas J; Kusters, J. G.; Stojiljkovic, I.; Heffron, F.

In: Infection and Immunity, Vol. 62, No. 5, 1994, p. 1623-1630.

Research output: Contribution to journalArticle

Baumler, AJ, Kusters, JG, Stojiljkovic, I & Heffron, F 1994, 'Salmonella typhimurium loci involved in survival within macrophages', Infection and Immunity, vol. 62, no. 5, pp. 1623-1630.
Baumler AJ, Kusters JG, Stojiljkovic I, Heffron F. Salmonella typhimurium loci involved in survival within macrophages. Infection and Immunity. 1994;62(5):1623-1630.
Baumler, Andreas J ; Kusters, J. G. ; Stojiljkovic, I. ; Heffron, F. / Salmonella typhimurium loci involved in survival within macrophages. In: Infection and Immunity. 1994 ; Vol. 62, No. 5. pp. 1623-1630.
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