Salmonella typhimurium enzymatically landscapes the host intestinal epithelial cell (IEC) surface glycome to increase invasion

Dayoung Park, Narine Arabyan, Cynthia C. Williams, Ting Song, Anupam Mitra, Bart C Weimer, Emanual Michael Maverakis, Carlito B Lebrilla

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Although gut host-pathogen interactions are glycan-mediated processes, few details are known about the participating structures. Here we employ high-resolution mass spectrometric profiling to comprehensively identify and quantitatively measure the exact modifications of native intestinal epithelial cell surface N-glycans induced by S. typhimurium infection. Sixty minutes postinfection, select sialylated structures showed decreases in terms of total number and abundances. To assess the effect of cell surface mannosylation, we selectively rerouted glycan expression on the host using the alpha-mannosidase inhibitor, kifunensine, toward overexpression of high mannose. Under these conditions, internalization of S. typhimurium significantly increased, demonstrating that bacteria show preference for particular structures. Finally, we developed a novel assay to measure membrane glycoprotein turnover rates, which revealed that glycan modifications occur by bacterial enzyme activity rather than by hostderived restructuring strategies. This study is the first to provide precise structural information on how host N-glycans are altered to support S. typhimurium invasion.

Original languageEnglish (US)
Pages (from-to)3653-3664
Number of pages12
JournalMolecular and Cellular Proteomics
Volume15
Issue number12
DOIs
StatePublished - Dec 1 2016

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Salmonella
Salmonella typhimurium
Polysaccharides
Epithelial Cells
alpha-Mannosidase
Host-Pathogen Interactions
Salmonella Infections
Membrane Glycoproteins
Enzyme activity
Pathogens
Mannose
Assays
Bacteria
Enzymes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology

Cite this

Salmonella typhimurium enzymatically landscapes the host intestinal epithelial cell (IEC) surface glycome to increase invasion. / Park, Dayoung; Arabyan, Narine; Williams, Cynthia C.; Song, Ting; Mitra, Anupam; Weimer, Bart C; Maverakis, Emanual Michael; Lebrilla, Carlito B.

In: Molecular and Cellular Proteomics, Vol. 15, No. 12, 01.12.2016, p. 3653-3664.

Research output: Contribution to journalArticle

Park, Dayoung ; Arabyan, Narine ; Williams, Cynthia C. ; Song, Ting ; Mitra, Anupam ; Weimer, Bart C ; Maverakis, Emanual Michael ; Lebrilla, Carlito B. / Salmonella typhimurium enzymatically landscapes the host intestinal epithelial cell (IEC) surface glycome to increase invasion. In: Molecular and Cellular Proteomics. 2016 ; Vol. 15, No. 12. pp. 3653-3664.
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