Abstract
Salmonella infection profoundly affects host erythroid development, but the mechanisms responsible for this effect remain poorly understood. We monitored the impact of Salmonella infection on erythroid development and found that systemic infection induced anemia, splenomegaly, elevated erythropoietin (EPO) levels, and extramedullary erythropoiesis in a process independent of Salmonella pathogenicity island 2 (SPI2) or flagellin. The circulating EPO level was also constitutively higher in mice lacking the expression of signal-regulatory protein α (SIRPα). The expression level of EPO mRNA was elevated in the kidney and liver but not increased in the spleens of infected mice despite the presence of extramedullary erythropoiesis in this tissue. In contrast to data from a previous report, mice lacking EPO receptor (EPOR) expression on nonerythroid cells (EPOR rescued) had bacterial loads similar to those of wild-type mice following Salmonella infection. Indeed, treatment to reduce splenic erythroblasts and mature red blood cells correlated with elevated bacterial burdens, implying that extramedullary erythropoiesis benefits the host. Together, these findings emphasize the profound effect of Salmonella infection on erythroid development and suggest that the modulation of erythroid development has both positive and negative consequences for host immunity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2833-2841 |
| Number of pages | 9 |
| Journal | Infection and Immunity |
| Volume | 84 |
| Issue number | 10 |
| DOIs | |
| State | Published - 2016 |
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ASJC Scopus subject areas
- Parasitology
- Microbiology
- Immunology
- Infectious Diseases
Cite this
Salmonella infection enhances erythropoietin production by the kidney and liver, which correlates with elevated bacterial burdens. / Li, Lin Xi; Benoun, Joseph M.; Weiskopf, Kipp; Garcia, K. Christopher; Mcsorley, Stephen J.
In: Infection and Immunity, Vol. 84, No. 10, 2016, p. 2833-2841.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Salmonella infection enhances erythropoietin production by the kidney and liver, which correlates with elevated bacterial burdens
AU - Li, Lin Xi
AU - Benoun, Joseph M.
AU - Weiskopf, Kipp
AU - Garcia, K. Christopher
AU - Mcsorley, Stephen J
PY - 2016
Y1 - 2016
N2 - Salmonella infection profoundly affects host erythroid development, but the mechanisms responsible for this effect remain poorly understood. We monitored the impact of Salmonella infection on erythroid development and found that systemic infection induced anemia, splenomegaly, elevated erythropoietin (EPO) levels, and extramedullary erythropoiesis in a process independent of Salmonella pathogenicity island 2 (SPI2) or flagellin. The circulating EPO level was also constitutively higher in mice lacking the expression of signal-regulatory protein α (SIRPα). The expression level of EPO mRNA was elevated in the kidney and liver but not increased in the spleens of infected mice despite the presence of extramedullary erythropoiesis in this tissue. In contrast to data from a previous report, mice lacking EPO receptor (EPOR) expression on nonerythroid cells (EPOR rescued) had bacterial loads similar to those of wild-type mice following Salmonella infection. Indeed, treatment to reduce splenic erythroblasts and mature red blood cells correlated with elevated bacterial burdens, implying that extramedullary erythropoiesis benefits the host. Together, these findings emphasize the profound effect of Salmonella infection on erythroid development and suggest that the modulation of erythroid development has both positive and negative consequences for host immunity.
AB - Salmonella infection profoundly affects host erythroid development, but the mechanisms responsible for this effect remain poorly understood. We monitored the impact of Salmonella infection on erythroid development and found that systemic infection induced anemia, splenomegaly, elevated erythropoietin (EPO) levels, and extramedullary erythropoiesis in a process independent of Salmonella pathogenicity island 2 (SPI2) or flagellin. The circulating EPO level was also constitutively higher in mice lacking the expression of signal-regulatory protein α (SIRPα). The expression level of EPO mRNA was elevated in the kidney and liver but not increased in the spleens of infected mice despite the presence of extramedullary erythropoiesis in this tissue. In contrast to data from a previous report, mice lacking EPO receptor (EPOR) expression on nonerythroid cells (EPOR rescued) had bacterial loads similar to those of wild-type mice following Salmonella infection. Indeed, treatment to reduce splenic erythroblasts and mature red blood cells correlated with elevated bacterial burdens, implying that extramedullary erythropoiesis benefits the host. Together, these findings emphasize the profound effect of Salmonella infection on erythroid development and suggest that the modulation of erythroid development has both positive and negative consequences for host immunity.
UR - http://www.scopus.com/inward/record.url?scp=84994302362&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84994302362&partnerID=8YFLogxK
U2 - 10.1128/IAI.00337-16
DO - 10.1128/IAI.00337-16
M3 - Article
C2 - 27456828
AN - SCOPUS:84994302362
VL - 84
SP - 2833
EP - 2841
JO - Infection and Immunity
JF - Infection and Immunity
SN - 0019-9567
IS - 10
ER -