Salmonella enterica serovar Typhimurium-induced internalization and IL-8 expression in HeLa cells does not have a direct relationship with intracellular Ca2+ levels

Josely F. Figueiredo, Rola Barhoumi, Manuela Raffatellu, Sara D. Lawhon, Bernard Rousseau, Robert C. Burghardt, Renee M Tsolis, Andreas J Baumler, L. Garry Adams

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The invasion-associated type III secretion system (T3SS-1) of S. Typhimurium is required to initiate and sustain an acute inflammatory response in the intestine. We investigated the relationship of S. Typhimurium T3SS-1-induced IL-8 expression and invasion with intracellular Ca2+ mobilization in HeLa cells. Compared to the sipAsopABDE2 mutant, strains carrying a mutation in sipA, or mutations in sopABDE2 induced higher levels of IL-8 and greater bacterial internalization despite the fact that these mutants elicited similarly low intracellular concentrations of Ca2+. Likewise, complemented sipAsopABDE2 mutant with sopE2 did not affect intracellular Ca2+ concentrations or IL-8 expression, but significantly increased bacterial internalization. Treating HeLa cells with the calcium chelator BAPTA-AM or with D-BAPTA-AM, a derivative with greatly reduced Ca2+ chelating activity, yielded strong evidence that BAPTA-AM does not affect invasion and inhibits IL-8 secretion by a calcium-dependent mechanism. These findings suggest that, although wild-type S. Typhimurium-induced IL-8 expression and bacterial internalization in HeLa cells coincides with increased cytosolic Ca2+, the differing levels of IL-8 and invasion induced by strains carrying different effector proteins are unrelated to levels of intracellular Ca2+.

Original languageEnglish (US)
Pages (from-to)850-858
Number of pages9
JournalMicrobes and Infection
Volume11
Issue number10-11
DOIs
StatePublished - Sep 2009

Fingerprint

Salmonella enterica
Interleukin-8
HeLa Cells
Mutation
Intestines
Serogroup
Calcium
1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester
Proteins

Keywords

  • Calcium
  • IL-8
  • Salmonella enterica serovar Typhimurium
  • SipA
  • T3SS-1

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Salmonella enterica serovar Typhimurium-induced internalization and IL-8 expression in HeLa cells does not have a direct relationship with intracellular Ca2+ levels. / Figueiredo, Josely F.; Barhoumi, Rola; Raffatellu, Manuela; Lawhon, Sara D.; Rousseau, Bernard; Burghardt, Robert C.; Tsolis, Renee M; Baumler, Andreas J; Adams, L. Garry.

In: Microbes and Infection, Vol. 11, No. 10-11, 09.2009, p. 850-858.

Research output: Contribution to journalArticle

Figueiredo, Josely F. ; Barhoumi, Rola ; Raffatellu, Manuela ; Lawhon, Sara D. ; Rousseau, Bernard ; Burghardt, Robert C. ; Tsolis, Renee M ; Baumler, Andreas J ; Adams, L. Garry. / Salmonella enterica serovar Typhimurium-induced internalization and IL-8 expression in HeLa cells does not have a direct relationship with intracellular Ca2+ levels. In: Microbes and Infection. 2009 ; Vol. 11, No. 10-11. pp. 850-858.
@article{55bf28ac9ee84beb8564bec8eb54c958,
title = "Salmonella enterica serovar Typhimurium-induced internalization and IL-8 expression in HeLa cells does not have a direct relationship with intracellular Ca2+ levels",
abstract = "The invasion-associated type III secretion system (T3SS-1) of S. Typhimurium is required to initiate and sustain an acute inflammatory response in the intestine. We investigated the relationship of S. Typhimurium T3SS-1-induced IL-8 expression and invasion with intracellular Ca2+ mobilization in HeLa cells. Compared to the sipAsopABDE2 mutant, strains carrying a mutation in sipA, or mutations in sopABDE2 induced higher levels of IL-8 and greater bacterial internalization despite the fact that these mutants elicited similarly low intracellular concentrations of Ca2+. Likewise, complemented sipAsopABDE2 mutant with sopE2 did not affect intracellular Ca2+ concentrations or IL-8 expression, but significantly increased bacterial internalization. Treating HeLa cells with the calcium chelator BAPTA-AM or with D-BAPTA-AM, a derivative with greatly reduced Ca2+ chelating activity, yielded strong evidence that BAPTA-AM does not affect invasion and inhibits IL-8 secretion by a calcium-dependent mechanism. These findings suggest that, although wild-type S. Typhimurium-induced IL-8 expression and bacterial internalization in HeLa cells coincides with increased cytosolic Ca2+, the differing levels of IL-8 and invasion induced by strains carrying different effector proteins are unrelated to levels of intracellular Ca2+.",
keywords = "Calcium, IL-8, Salmonella enterica serovar Typhimurium, SipA, T3SS-1",
author = "Figueiredo, {Josely F.} and Rola Barhoumi and Manuela Raffatellu and Lawhon, {Sara D.} and Bernard Rousseau and Burghardt, {Robert C.} and Tsolis, {Renee M} and Baumler, {Andreas J} and Adams, {L. Garry}",
year = "2009",
month = "9",
doi = "10.1016/j.micinf.2009.05.003",
language = "English (US)",
volume = "11",
pages = "850--858",
journal = "Microbes and Infection",
issn = "1286-4579",
publisher = "Elsevier Masson SAS",
number = "10-11",

}

TY - JOUR

T1 - Salmonella enterica serovar Typhimurium-induced internalization and IL-8 expression in HeLa cells does not have a direct relationship with intracellular Ca2+ levels

AU - Figueiredo, Josely F.

AU - Barhoumi, Rola

AU - Raffatellu, Manuela

AU - Lawhon, Sara D.

AU - Rousseau, Bernard

AU - Burghardt, Robert C.

AU - Tsolis, Renee M

AU - Baumler, Andreas J

AU - Adams, L. Garry

PY - 2009/9

Y1 - 2009/9

N2 - The invasion-associated type III secretion system (T3SS-1) of S. Typhimurium is required to initiate and sustain an acute inflammatory response in the intestine. We investigated the relationship of S. Typhimurium T3SS-1-induced IL-8 expression and invasion with intracellular Ca2+ mobilization in HeLa cells. Compared to the sipAsopABDE2 mutant, strains carrying a mutation in sipA, or mutations in sopABDE2 induced higher levels of IL-8 and greater bacterial internalization despite the fact that these mutants elicited similarly low intracellular concentrations of Ca2+. Likewise, complemented sipAsopABDE2 mutant with sopE2 did not affect intracellular Ca2+ concentrations or IL-8 expression, but significantly increased bacterial internalization. Treating HeLa cells with the calcium chelator BAPTA-AM or with D-BAPTA-AM, a derivative with greatly reduced Ca2+ chelating activity, yielded strong evidence that BAPTA-AM does not affect invasion and inhibits IL-8 secretion by a calcium-dependent mechanism. These findings suggest that, although wild-type S. Typhimurium-induced IL-8 expression and bacterial internalization in HeLa cells coincides with increased cytosolic Ca2+, the differing levels of IL-8 and invasion induced by strains carrying different effector proteins are unrelated to levels of intracellular Ca2+.

AB - The invasion-associated type III secretion system (T3SS-1) of S. Typhimurium is required to initiate and sustain an acute inflammatory response in the intestine. We investigated the relationship of S. Typhimurium T3SS-1-induced IL-8 expression and invasion with intracellular Ca2+ mobilization in HeLa cells. Compared to the sipAsopABDE2 mutant, strains carrying a mutation in sipA, or mutations in sopABDE2 induced higher levels of IL-8 and greater bacterial internalization despite the fact that these mutants elicited similarly low intracellular concentrations of Ca2+. Likewise, complemented sipAsopABDE2 mutant with sopE2 did not affect intracellular Ca2+ concentrations or IL-8 expression, but significantly increased bacterial internalization. Treating HeLa cells with the calcium chelator BAPTA-AM or with D-BAPTA-AM, a derivative with greatly reduced Ca2+ chelating activity, yielded strong evidence that BAPTA-AM does not affect invasion and inhibits IL-8 secretion by a calcium-dependent mechanism. These findings suggest that, although wild-type S. Typhimurium-induced IL-8 expression and bacterial internalization in HeLa cells coincides with increased cytosolic Ca2+, the differing levels of IL-8 and invasion induced by strains carrying different effector proteins are unrelated to levels of intracellular Ca2+.

KW - Calcium

KW - IL-8

KW - Salmonella enterica serovar Typhimurium

KW - SipA

KW - T3SS-1

UR - http://www.scopus.com/inward/record.url?scp=68849109413&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=68849109413&partnerID=8YFLogxK

U2 - 10.1016/j.micinf.2009.05.003

DO - 10.1016/j.micinf.2009.05.003

M3 - Article

C2 - 19450704

AN - SCOPUS:68849109413

VL - 11

SP - 850

EP - 858

JO - Microbes and Infection

JF - Microbes and Infection

SN - 1286-4579

IS - 10-11

ER -