Salmonella enterica serotype Typhimurium MisL is an intestinal colonization factor that binds fibronectin

Caleb W. Dorsey, Marijke C. Laarakker, Andrea D. Humphries, Eric H. Weening, Andreas J Baumler

Research output: Contribution to journalArticle

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Abstract

MisL is an autotransporter protein encoded by Salmonella pathogenicity island 3 (SPI3). To investigate the role of MisL in Salmonella enterica serotype Typhimurium (S. Typhimurium) pathogenesis, we characterized its function during infection of mice and identified a host receptor for this adhesin. In a mouse model of S. Typhimurium intestinal persistence, a misL mutant was shed with the faeces in significantly lower numbers than the wild type and was impaired in its ability to colonize the cecum. Previous studies have implicated binding of extracellular matrix proteins as a possible mechanism for S. Typhimurium intestinal persistence. A gluthathione-S-transferase (GST) fusion protein to the MisL passenger domain (GST-MisL29-281) was constructed to investigate binding to extracellular matrix proteins. In a solid-phase binding assay the purified GST-MisL29-281 fusion protein bound to fibronectin and collagen IV, but not to collagen I. MisL expression was not detected by Western blot in S. Typhimurium grown under standard laboratory conditions. However, when expression of the cloned misL. gene was driven by the Escherichia coli arabinose promoter, MisL could be detected in the S. Typhimurium outer membrane by Western blot and on the bacterial cell surface by flow cytometry. Expression of MisL enabled S. Typhimurium to bind fibronectin to its cell surface, resulting in attachment to fibronectin-coated glass slides and in increased invasiveness for human epithelial cells derived from colonic carcinoma (T84 cells). These data identify MisL as an extracellular matrix adhesin involved in intestinal colonization.

Original languageEnglish (US)
Pages (from-to)196-211
Number of pages16
JournalMolecular Microbiology
Volume57
Issue number1
DOIs
StatePublished - Jul 2005

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Salmonella enterica
Fibronectins
Glutathione Transferase
Extracellular Matrix Proteins
Collagen
Western Blotting
Genomic Islands
Arabinose
Proteins
Cecum
Feces
Salmonella
Glass
Extracellular Matrix
Serogroup
Flow Cytometry
Epithelial Cells
Escherichia coli
Carcinoma
Membranes

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Salmonella enterica serotype Typhimurium MisL is an intestinal colonization factor that binds fibronectin. / Dorsey, Caleb W.; Laarakker, Marijke C.; Humphries, Andrea D.; Weening, Eric H.; Baumler, Andreas J.

In: Molecular Microbiology, Vol. 57, No. 1, 07.2005, p. 196-211.

Research output: Contribution to journalArticle

Dorsey, Caleb W. ; Laarakker, Marijke C. ; Humphries, Andrea D. ; Weening, Eric H. ; Baumler, Andreas J. / Salmonella enterica serotype Typhimurium MisL is an intestinal colonization factor that binds fibronectin. In: Molecular Microbiology. 2005 ; Vol. 57, No. 1. pp. 196-211.
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